Human LRP-1 Cluster III Antibody

Catalog #: AF4824 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4824
AF4824-SP

LRP‑1 Cluster III in MG‑63 Human Cell Line.

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Human LRP-1 Cluster III Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRP-1 Cluster III in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived human LRP-1 Cluster III
Ser2522-Ile2941
Accession # Q07954

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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LRP‑1 Cluster III in MG‑63 Human Cell Line. LRP-1 Cluster III was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human LRP-1 Cluster III Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1 Cluster III

LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled‑1, various integrins, and the prion protein PrP^C (1, 5‑10). Human LRP-1 is an N‑glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane beta chain and a 515 kDa alpha chain that associates noncovalently with the beta chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF‑like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). LRP-1 Cluster III (aa 2522‑2941) contains ten LDLR-A cysteine-rich domains (14). Within this region, human LRP-1 shares 97% aa sequence identity with mouse and rat LRP-1. A soluble form of LRP-1 is shed into the serum and cerebrospinal fluid and retains ligand binding properties (15, 16). LRP-1 Cluster III contains binding sites for LRPAP/RAP (14).

References

Lillis, A.P. et al. (2008) Physiol. Rev. 88:887.
Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729.
Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638.
Sendra, J. et al. (2008) Cardiovasc. Res. 78:581.
Muratoglu, S.C. et al. (2010) J. Biol. Chem. 258:14308.
Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004.
Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17.
Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773.
Herz, J. et al. (1990) EMBO J. 9:1769.
Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401.
Herz, J. et al. (1988) EMBO J. 7:4119.
Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305.
Liu, Q. et al. (2009) Mol. Neurodegener. 4:17.
Gorovoy, M. et al. (2010) J. Leukoc. Biol. Jul 7 epub.

Long Name

LDL Receptor-related Protein 1, Cluster III

Entrez Gene IDs

4035 (Human); 16971 (Mouse); 299858 (Rat)

Alternate Names

A2MR;APOER;APR;CD91;IGFBP3R;IGFBP-3R;IGFBP3R1;KPA;LRP;LRP1A;Prolow-density lipoprotein receptor-related protein 1;TGFBR5; LRP1 Cluster III; LRP-1 Cluster III