Human LRRC3 Antibody

Catalog #: AF5039 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5039-SP	Ships in 5-7 Days	25 ug / $149
AF5039	In Stock	100 ug / $469

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Human LRRC3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRRC3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human LRRC4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRRC3
Cys33-Asp204
Accession # Q9BY71

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LRRC3

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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LRRC3 in Human Brain. LRRC3 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human LRRC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5039) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC3

LRRC3 (Leu-rich repeat/LRR-containing protein 3) is a 25 kDa (predicted) member of the Leu-rich repeat protein superfamily. Little is known about the protein except for the fact that it shows widespread expression. Human LRRC3 is a protein that is 225 amino acids (aa) in length. It contains three LRRs between aa 63‑86, aa 87‑110 and aa 112‑135. There is one potential splice variant that shows a deletion of aa 129‑248. Over aa 33‑204, human LRRC3 is 83% and 81% aa identical to mouse and canine LRRC3, respectively.

Long Name

Leucine-rich Repeat Containing 3

Entrez Gene IDs

81543 (Human); 237387 (Mouse); 246773 (Rat)

Alternate Names

C21orf102; chromosome 21 open reading frame 102; leucine rich repeat containing 3; leucine-rich repeat-containing protein 3; LRRC3; LRRC3A