Human LRRC3B Antibody

Catalog #: MAB7635 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7635
MAB7635-SP

Detection of Human LRRC3B by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRRC3B Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRRC3B in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 758315

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LRRC3B
Phe28-Ala205
Accession # Q96PB8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LRRC3B by Western Blot. Western blot shows lysates of human prostate tissue and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRRC3B Monoclonal Antibody (Catalog # MAB7635) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LRRC3B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC3B

LRRC3B, also known as LRP15, is a transmembrane leucine-rich protein that is downregulated by promoter methylation in gastric and colorectal cancers. It confers resistance to ultraviolet light-induced DNA damage and functions as a tumor suppressor. Mature human LRRC3B contains an LRR N-terminal domain (aa 34-64), 3 LRR repeats (aa 65-135), an LRR C-terminal domain (aa 145-197), a transmembrane segment (aa 205-225), and a C-terminal region (aa 226-259). Within aa 28-205, human LRRC3B shares 100% aa sequence identity with mouse and rat LRRC3B.

Long Name

Leucine Rich Repeat Containing 3B

Entrez Gene IDs

116135 (Human); 218763 (Mouse)

Alternate Names

leucine rich repeat containing 3B; leucine-rich repeat-containing protein 3B; LRP15; LRP15Leucine-rich repeat protein LRP15; LRRC3B; MGC102927