Human M-CSF Antibody

Catalog #: MAB216
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB216-SP
MAB216-100
MAB216-500

Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody.

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All M-CSF Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human M-CSF Antibody Summary

Species Reactivity

Human

Specificity

Detects human M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse M‑CSF.

Source

Monoclonal Mouse IgG_2A Clone # 26730

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human M-CSF

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human M-CSF (Catalog # 216-MC)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND₅₀) is typically 0.005-0.020 µg/mL in the presence of 2.5 ng/mL Recombinant Human M‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by M‑CSF and Neutralization by Human M‑CSF Antibody. Recombinant Human M‑CSF (Catalog # 216-MC) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human M‑CSF (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human M‑CSF Monoclonal Antibody (Catalog # MAB216). The ND₅₀ is typically 0.005-0.020 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name

Macrophage Colony Stimulating Factor

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Alternate Names

colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human M-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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CSF1/CSF1R-mediated Crosstalk Between Choroidal Vascular Endothelial Cells and Macrophages Promotes Choroidal Neovascularization
Authors: Yamei Zhou, Jia Zeng, Yuanyuan Tu, Lele Li, Shu Du, Linling Zhu et al.
Investigative Opthalmology & Visual Science
Signal Integration and Transcriptional Regulation of the Inflammatory Response Mediated by the GM-/M-CSF Signaling Axis in Human Monocytes
Authors: RM Rodriguez, B Suarez-Alv, JL Lavín, AM Ascensión, M Gonzalez, JJ Lozano, AB Raneros, PD Bulnes, JR Vidal-Cast, C Huidobro, C Martin-Mar, AB Sanz, M Ruiz-Orteg, A Puig-Kröge, AL Corbí, MJ Araúzo-Bra, AM Aransay, C Lopez-Larr
Cell Rep, 2019-10-22;29(4):860-872.e5.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
Blood, 2007-09-11;110(13):4319-30.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization