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Human M-CSF ELISA Kit - Quantikine

Catalog #: DMC00B
35 Citations Datasheet / COA / SDS
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QC21: Quantikine Immunoassay Control Group 4
Human M-CSF ELISA Cell Culture Supernates/Saliva/Urine Standard Curve
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Citations (35)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human M-CSF Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (100 uL), Urine (100 uL)
Sensitivity
47.3 pg/mL
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human M-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human M-CSF in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human M-CSF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human M-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human M-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 248 751 1503 280 827 1620
Standard Deviation 19.4 16.1 49 30.2 76 87.7
CV% 7.8 2.1 3.3 10.7 9.2 5.4

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 357 951 1767 343 1019 1956
Standard Deviation 17.4 24.9 49.4 43.4 84.1 88.8
CV% 4.9 2.6 2.8 12.6 8.3 4.5

Recovery

The recovery of M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 90-107
EDTA Plasma (n=4) 100 93-106
Heparin Plasma (n=4) 99 93-106
Serum (n=4) 101 96-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of M-CSF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human M-CSF ELISA Linearity

Scientific Data

N/A Human M-CSF ELISA Cell Culture Supernates/Saliva/Urine Standard Curve View Larger

Human M-CSF ELISA Cell Culture Supernates/Saliva/Urine Standard Curve

N/A Human M-CSF ELISA Serum/Plasma Standard Curve View Larger

Human M-CSF ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name:
Macrophage Colony Stimulating Factor
Entrez Gene IDs:
1435 (Human); 12977 (Mouse)
Alternate Names:
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human M-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis
    Authors: JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan
    Cell Death & Disease, 2022-06-06;13(6):527.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. M-CSF supports medullary erythropoiesis and erythroid iron demand following burn injury through its activity on homeostatic iron recycling
    Authors: JG Noel, SW Ramser, L Pitstick, JP Bonamer, B Mackenzie, KG Seu, TA Kalfa, JA Cancelas, JC Gardner
    Scientific Reports, 2022-01-24;12(1):1235.
    Species: Human
    Sample Types: Plasma
  3. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
    Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
    Antioxidants (Basel), 2021-05-28;10(6):.
    Species: Human
    Sample Types: Urine
  4. Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis
    Authors: R Samain, A Brunel, T Douché, M Fanjul, S Cassant-So, J Rochotte, J Cros, C Neuzillet, J Raffenne, C Duluc, A Perraud, J Nigri, V Gigoux, I Bieche, M Ponzo, G Carpentier, I Cascone, R Tomasini, HA Schmid, M Mathonnet, R Nicolle, MP Bousquet, Y Martineau, S Pyronnet, C Jean, C Bousquet
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Co-Overexpression of TWIST1-CSF1 Is a Common Event in Metastatic Oral Cancer and Drives Biologically Aggressive Phenotype
    Authors: SD da Silva, FA Marchi, J Su, L Yang, L Valverde, J Hier, K Bijian, M Hier, A Mlynarek, LP Kowalski, MA Alaoui-Jam
    Cancers, 2021-01-05;13(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. CSF1R signaling is a regulator of pathogenesis in progressive MS
    Authors: N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim
    Cell Death Dis, 2020-10-23;11(10):904.
    Species: Human
    Sample Types: Cell Culture Supernates, CSF
  7. FOXO1 promotes tumor progression by increased M2 macrophage infiltration in esophageal squamous cell carcinoma
    Authors: Y Wang, Z Lyu, Y Qin, X Wang, L Sun, Y Zhang, L Gong, S Wu, S Han, Y Tang, Y Jia, DL Kwong, N Kam, XY Guan
    Theranostics, 2020-09-16;10(25):11535-11548.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study
    Authors: S Ma, T Tominaga, K Kanda, K Sugama, C Omae, S Hashimoto, K Aoyama, Y Yoshikai, K Suzuki
    Biomedicines, 2020-03-04;8(3):.
    Species: Human
    Sample Types: Urine
  9. ERK1/2 signalling regulates the immune microenvironment and macrophage recruitment in glioblastoma
    Authors: C Lailler, C Louandre, MC Morisse, T Lhossein, C Godin, M Lottin, JM Constans, B Chauffert, A Galmiche, Z Saidak
    Biosci. Rep., 2019-09-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Multilevel defects in the hematopoietic niche in essential thrombocythemia
    Authors: T Sun, M Ju, X Dai, H Dong, W Gu, Y Gao, R Fu, X Liu, Y Huang, W Liu, Y Chi, W Wang, H Li, Y Zhou, L Shi, R Yang, L Zhang
    Haematologica, 2019-07-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment
    Authors: J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park
    Sci Rep, 2019-06-19;9(1):8726.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Plasma levels of M-CSF and VEGF in laboratory diagnostics and differentiation of selected histological types of cervical cancers
    Authors: I Sidorkiewi, M Zbucka-Kr?, K Zar?ba, E Lubowicka, M Zajkowska, M Szmitkowsk, E Gacuta, S ?awicki
    BMC Cancer, 2019-04-29;19(1):398.
    Species: Human
    Sample Types: Plasma
  13. Effects of loxoprofen on the apical root resorption during orthodontic tooth movement in rats
    Authors: T Yamamoto, M Kaku, H Sumi, Y Yashima, J Izumino, K Tanimoto
    PLoS ONE, 2018-04-25;13(4):e0194453.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Serum bone-turnover biomarkers are associated with the occurrence of peripheral and axial arthritis in psoriatic disease: a prospective cross-sectional comparative study
    Authors: DR Jadon, R Sengupta, A Nightingal, H Lu, J Dunphy, A Green, JT Elder, RP Nair, E Korendowyc, MA Lindsay, NJ McHugh
    Arthritis Res. Ther., 2017-09-21;19(1):210.
    Species: Human
    Sample Types: Serum
  15. New Markers of Inflammation and Tubular Damage in Children with Chronic Kidney Disease
    Authors: K Musia?, A Bargenda, D Dro?d?, D Zwoli?ska
    Dis. Markers, 2017-07-20;2017(0):9389432.
    Species: Human
    Sample Types: Urine
  16. Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines
    Authors: S Pourteymou, K Eckardt, T Holen, T Langleite, S Lee, J Jensen, KI Birkeland, CA Drevon, M Hjorth
    Mol Metab, 2017-01-29;6(4):352-365.
    Species: Human
    Sample Types: Plasma
  17. Effect of low-level laser therapy on bisphosphonate-treated osteoblasts
    Authors: Sang-Hun Shin
    Maxillofac Plast Reconstr Surg, 2016-11-25;38(1):48.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Pleural Effusions from Patients with Mesothelioma Induce Recruitment of Monocytes and Their Differentiation into M2 Macrophages.
    Authors: Chene A, d'Almeida S, Blondy T, Tabiasco J, Deshayes S, Fonteneau J, Cellerin L, Delneste Y, Gregoire M, Blanquart C
    J Thorac Oncol, 2016-07-12;11(10):1765-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Urokinase receptor mediates osteoclastogenesis via M-CSF release from osteoblasts and the c-Fms/PI3K/Akt/NF-kappaB pathway in osteoclasts.
    Authors: Kalbasi Anaraki P, Patecki M, Tkachuk S, Kiyan Y, Haller H, Dumler I
    J Bone Miner Res, 2015-02-01;30(2):379-88.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
    Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
    Blood, 2012-09-18;120(19):4058-67.
    Species: Human
    Sample Types: Serum
  21. Induced osteoclastogenesis by fluoroquinolones in unstimulated and stimulated human osteoclast precursor cells.
    Authors: Costa-Rodrigues J, Martins EG, Fernandes MH
    Bone, 2012-03-23;51(1):17-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
    Authors: Kreutz M, Karrer S, Hoffmann P
    J. Invest. Dermatol., 2011-08-18;132(1):179-87.
    Species: Human
    Sample Types: Serum
  23. Hypoxia potentiates glioma-mediated immunosuppression.
    Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
    PLoS ONE, 2011-01-20;6(1):e16195.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation.
    Authors: Seizer P, Schonberger T, Schott M, Lang MR, Langer HF, Bigalke B, Kramer BF, Borst O, Daub K, Heidenreich O, Schmidt R, Lindemann S, Herouy Y, Gawaz M, May AE
    Atherosclerosis, 2009-08-21;209(1):51-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009-01-12;206(1):169-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Circulating level of macrophage colony-stimulating factor can be predictive for human in vitro fertilization outcome.
    Authors: Salmassi A, Mettler L, Jonat W
    Fertil. Steril., 2008-11-08;93(1):116-23.
    Species: Human
    Sample Types: Serum
  27. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide.
    Authors: Dower K, Ellis DK, Saraf K, Jelinsky SA, Lin LL
    J. Immunol., 2008-03-01;180(5):3520-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Exogenous heat shock protein 27 uniquely blocks differentiation of monocytes to dendritic cells.
    Authors: Laudanski K, De A, Miller-Graziano C
    Eur. J. Immunol., 2007-10-01;37(10):2812-24.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. The cell-surface isoform of colony stimulating factor 1 (CSF1) restores but does not completely normalize fecundity in CSF1-deficient mice.
    Authors: Ovadia S, Insogna K, Yao GQ
    Biol. Reprod., 2005-10-19;74(2):331-6.
    Species: Human
    Sample Types: Tissue Homogenates
  31. Markers of immune activation and viral load in HIV-associated sensory neuropathy.
    Authors: Schifitto G, McDermott MP, McArthur JC, Marder K, Sacktor N, McClernon DR, Conant K, Cohen B, Epstein LG, Kieburtz K, Kieburtz K
    Neurology, 2005-03-08;64(5):842-8.
    Species: Human
    Sample Types: Plasma
  32. The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells.
    Authors: Kawano Y, Fukuda J, Itoh H, Takai N, Nasu K, Miyakawa I
    Am. J. Reprod. Immunol., 2004-08-01;52(2):124-8.
    Species: Human
    Sample Types: Follicular Fluid
  33. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004-02-01;55(2):221-35.
    Species: Human
    Sample Types: CSF
  34. Distinct hematopoietic support by two human stromal cell lines.
    Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
    Exp. Hematol., 2001-06-01;29(6):736-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. High macrophage-colony stimulating factor levels in synovial fluid of loose artificial hip joints.
    Authors: Takei I, Takagi M, Ida H, Ogino T, Santavirta S, Konttinen YT
    J. Rheumatol., 2000-04-01;27(4):894-9.
    Species: Human
    Sample Types: Synovial Fluid

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