Human MAP2 Antibody Summary
Gly1689-Lys1824
Accession # P11137
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of MAP2 in Human Brain Cortex via seqIF™ staining on COMET™ MAP2 Antibody was detected in immersion fixed paraffin-embedded sections of human Brain Cortex using Mouse Anti-Human MAP2, Monoclonal Antibody (Catalog # MAB8304) at 20ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
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MAP2 in Human Embryonic Stem Cells. MAP2 was detected in immersion fixed human embryonic stem cells/neurospheres using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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MAP2 in Human Brain. MAP2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human MAP2 Monoclonal Antibody (Catalog # MAB8304) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurofibrillary tangles and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAP2
MAP2 (Microtubule-associated protein 2) is a 1827 amino acid (aa) cytoskeletal associated protein. Human MAP2 shares 84% and 79% aa identity with mouse and rat MAP2, respectively. Multiple splice forms exist, resulting in 4 distinct isoforms. MAP2 functions in microtubule polymerization and stabilization in both normal and malignant cell types. It has been demonstrated to play a critical role in neurite outgrowth and loss of MAP2 function in neurons may lead to neural degeneration.
Product Datasheets
Citations for Human MAP2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons
Authors: Kuroda, T;Yasuda, S;Matsuyama, S;Miura, T;Sawada, R;Matsuyama, A;Yamamoto, Y;Morioka, MS;Kawaji, H;Kasukawa, T;Itoh, M;Akutsu, H;Kawai, J;Sato, Y;
Scientific reports
Species: Human
Sample Types: Whole Cells, Transduced Whole Cells
Applications: Immunocytochemistry -
Cell Specificity of Manganese-enhanced MRI Signal in the Cerebellum
Authors: Rallapalli, H;Bayin, NS;Goldman, H;Maric, D;Nieman, BJ;Koretsky, AP;Joyner, AL;Turnbull, DH;
NeuroImage
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
CXCL13 expressed on inflamed cerebral blood vessels recruit IL-21 producing TFH cells to damage neurons following stroke
Authors: Aditya Rayasam, Julie A. Kijak, Lee Kissel, Yun Hwa Choi, Taehee Kim, Martin Hsu et al.
Journal of Neuroinflammation
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NRSF/REST lies at the intersection between epigenetic regulation, miRNA-mediated gene control and neurodevelopmental pathways associated with Intellectual disability (ID) and Schizophrenia
Authors: Mouhamed Alsaqati, Brittany A. Davis, Jamie Wood, Megan M. Jones, Lora Jones, Aishah Westwood et al.
Translational Psychiatry
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Evidence of shared transcriptomic dysregulation of HNRNPU-related disorder between human organoids and embryonic mice
Authors: Andrew K. Ressler, Gabriela L.A. Sampaio, Sarah A. Dugger, Tamar Sapir, Daniel Krizay, Michael J. Boland et al.
iScience
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