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Human MBP Antibody

Catalog #: MAB42281 Datasheet / COA / SDS

Discontinued Product

MAB42281 has been discontinued.
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Detection of Human MBP by Western Blot.
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Human MBP Antibody Summary

Species Reactivity
Human
Specificity
Detects human MBP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 858901
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MBP
Gly2-Val133
Accession # P02686
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MBP antibody by Western Blot. View Larger

Detection of Human MBP by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MBP Monoclonal Antibody (Catalog # MAB42281) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MBP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBP

Myelin basic protein (MBP) is an abundant protein in the central nervous system. It is produced by oligodendrocytes and binds to cytoskeletal components and negatively charged lipids. These interactions enable MBP to anchor adjacent layers of myelin which oligodendocytes wrap around neuronal axons. Multiple sclerosis (MS) is a demyelinating inflammatory disease which is characterized by MBP with aberrant post-translational modifications, autoimmune reactions that target MBP, and oligodendrocyte apoptosis. Alternative splicing of human MBP generates several isoforms which lack amino acids 1-133, lack aa 240-250, and/or have a 26 aa insertion following Lys192, yielding protein products of 17-33 kDa. Within aa 1-133, human MBP shares 79% aa sequence identity with mouse MBP.

Long Name
Myelin Basic Protein
Entrez Gene IDs
4155 (Human); 17196 (Mouse); 24547 (Rat)
Alternate Names
Hmbpr; MBP; MGC99675; mld; Myelin A1 protein; myelin basic protein; Myelin membrane encephalitogenic protein; shi

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