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Human MCAM/CD146 Antibody

Catalog #: MAB932
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB932-SP
MAB932-500
MAB932-100
Detection of Human MCAM/CD146 by Western Blot.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCAM/CD146 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no  cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 128018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MCAM
Val24-Gly559
Accession # AAA20824
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MCAM/CD146 antibody by Western Blot. View Larger

Detection of Human MCAM/CD146 by Western Blot. Western blot shows lysate of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCAM/CD146 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of MCAM/CD146 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry MCAM/CD146 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

MCAM/CD146 in BG01V Human Embryonic Stem Cells. MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCAM/CD146

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

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Citations for Human MCAM/CD146 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Appearance of peanut agglutinin in the blood circulation after peanut ingestion promotes endothelial secretion of metastasis-promoting cytokines
    Authors: Weikun Wang, Paulina Sindrewicz-Goral, Chen Chen, Carrie A Duckworth, David Mark Pritchard, Jonathan M Rhodes et al.
    Carcinogenesis
  2. Precise CCM1 gene correction and inactivation in patient‐derived endothelial cells: Modeling Knudson's two‐hit hypothesis in vitro
    Authors: Stefanie Spiegler, Matthias Rath, Christiane D. Much, Barbara S. Sendtner, Ute Felbor
    Molecular Genetics & Genomic Medicine
  3. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts
    Authors: Costa D, Vene R, Benelli R et al.
    Front Immunol
  4. Proteomics profiling identifies extracellular vesicles' cargo associated with tumour cell induced platelet aggregation
    Authors: N McNamee, LR de la Fuen, MJ Santos-Mar, L O'Driscoll
    BMC Cancer, 2022-09-29;22(1):1023.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
    Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
    Sci Adv, 2020-02-26;6(9):eaax3223.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles
    Authors: MP Zaborowski, K Lee, YJ Na, A Sammarco, X Zhang, M Iwanicki, PS Cheah, HY Lin, M Zinter, CY Chou, G Fulci, BA Tannous, CP Lai, MJ Birrer, R Weissleder, H Lee, XO Breakefiel
    Cell Rep, 2019-04-02;27(1):255-268.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019-02-01;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems
    Authors: J Wang, W Anderson, J Li, LL Lin, Y Wang, M Trau
    J Colloid Interface Sci, 2018-11-12;537(0):536-546.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Dynamic Light Scattering
  9. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016-10-20;2016(0):6104780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Choice of xenogenic-free expansion media significantly influences the myogenic differentiation potential of human bone marrow-derived mesenchymal stromal cells.
    Authors: Brun J, Abruzzese T, Rolauffs B, Aicher W, Hart M
    Cytotherapy, 2016-03-01;18(3):344-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor.
    Authors: Im, Hyungsoo, Shao, Huilin, Park, Yong Il, Peterson, Vanessa, Castro, Cesar M, Weissleder, Ralph, Lee, Hakho
    Nat Biotechnol, 2014-04-20;32(5):490-5.
    Applications: Antibody Profiling
  12. Characterization of novel neutralizing mouse monoclonal antibody JM1-24-3 developed against MUC18 in metastatic melanoma
    Authors: Runhua Feng, Yuling Wang, Vijaya Ramachandran, Qinhong Ma, Matthew M. May, Ming Li et al.
    Journal of Experimental & Clinical Cancer Research
  13. Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells
    Authors: Ralph El Hallal, Nana Lyu, Yuling Wang
    Molecules

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