Home / MCAM/CD146 / Human MCAM/CD146 Fluorescein-conjugated Antibody

Human MCAM/CD146 Fluorescein-conjugated Antibody

Catalog #: FAB932F
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB932F-100
FAB932F-025
Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCAM/CD146 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm)OCAM, or rmMAdCAM-1.
Source
Monoclonal Mouse IgG1 Clone # 128018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
Val24-Gly559
Accession # AAA20824
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MCAM/CD146 antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB932F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

MCAM, also known as CD146 and MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

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Citations for Human MCAM/CD146 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019-11-04;10(1):316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
    Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
    PLoS ONE, 2014-01-29;9(1):e84417.
  3. Late outgrowth endothelial cells resemble mature endothelial cells and are not derived from bone marrow.
    Authors: Tura O, Skinner E, Barclay G, Samuel K, Gallagher R, Brittan M, Hadoke P, Newby D, Turner M, Mills N
    Stem Cells, 2013-02-01;31(2):338-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endothelial reconstitution by CD34+ progenitors derived from baboon embryonic stem cells
    Authors: Qiang Shi, Gerald Schatten, Vida Hodara, Calvin Simerly, John L. VandeBerg
    Journal of Cellular and Molecular Medicine
  5. Oxygen tension modifies the 'stemness' of human cord blood-derived stem cells.
    Authors: Buchheiser A, Houben A, Bosch J, Marbach J, Liedtke S, Kogler G
    Cytotherapy, 2012-04-12;14(8):967-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway.
    Authors: Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, Liu L, Jin Y
    J. Bone Miner. Res., 2011-09-01;26(9):2082-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Hyperoxia disrupts vascular endothelial growth factor-nitric oxide signaling and decreases growth of endothelial colony-forming cells from preterm infants.
    Authors: Fujinaga H, Baker CD, Ryan SL, Markham NE, Seedorf GJ, Balasubramaniam V, Abman SH
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-09-04;297(6):L1160-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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