Human Mesothelin Antibody Summary
Glu296-Gly580
Accession # AAH09272
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Mesothelin DuoSet ELISA Kit (Catalog # DY3265) for convenient development of a sandwich ELISA or the Human Mesothelin Quantikine ELISA Kit (Catalog # DMSLN0) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).
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Mesothelin Specificity is Shown by Flow Cytometry in Knockout Cell Line Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B). No staining in the Mesothelin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Human Mesothelin ELISA Standard Curve. Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mesothelin
Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.
Product Datasheets
Citations for Human Mesothelin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Mass Cytometry -
Activated iNKT cells enhance the anti-tumor effect of antigen specific CD8 T cells on mesothelin-expressing salivary gland cancer
Authors: Y Makita, N Kunii, D Sakurai, F Ihara, S Motohashi, A Suzuki, T Nakayama, Y Okamoto
BMC Cancer, 2018-12-17;18(1):1254.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Development of anti-human mesothelin-targeted chimeric antigen receptor (CAR) messenger RNA (mRNA)-transfected peripheral blood lymphocytes (CARMA-hMeso) for ovarian cancer therapy
Authors: CF Hung, X Xu, L Li, Y Ma, Q Jin, A Viley, C Allen, P Natarajan, R Shivakumar, MV Peshwa, LA Emens
Hum. Gene Ther., 2018-04-02;0(0):.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: CAR-T (Flow Cytometry)
FAQs
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Used the antibody at 1:100 dilution to stain HeLa cells. The secondary antibody was Rat F(ab)2 IgG (H+L) PE-conjugated antibody. Pink is the isotype control stained sample, and blue is the MSLN antibody stained sample. Iso control is Rat IgG2A.
Detection of Mesothelin on NCI-H226 lung squamous cell carcinoma cells. NCI-H226 cells were treated with 67 nM of the Human Mesothelin Antibody (catalog # MAB32652) or rat IgG2a isotype control, followed by a secondary APC AffiniPure F(ab')₂ Fragment Goat Anti-Rat IgG, F(ab')₂ fragment specific . Isotype control (RED), human TROP2 antibody (Blue).
