Home / Methionine Aminopeptidase 2/METAP2 / Human Methionine Aminopeptidase 2/
METAP2 Antibody

Human Methionine Aminopeptidase 2/
METAP2 Antibody

Catalog #: AF3795
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3795
AF3795-SP
Detection of Human Methionine Aminopeptidase 2 by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Methionine Aminopeptidase 2/
METAP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Methionine Aminopeptidase 2/METAP2 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Aminopeptidase 2/METAP2
Ala2-Tyr478
Accession # P50579
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Methionine Aminopeptidase 2/METAP2 (Catalog # 3795-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Methionine Aminopeptidase 2 antibody by Western Blot. View Larger

Detection of Human Methionine Aminopeptidase 2 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, LS180 human colorectal adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Methionine Aminopeptidase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3795) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Methionine Aminopeptidase 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 2/METAP2

The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.

References
  1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
  2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  3. Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.
Entrez Gene IDs
10988 (Human); 56307 (Mouse); 64370 (Rat)
Alternate Names
Amp2; eIF-2-associated p67 homolog; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; METAP2; Methionine Aminopeptidase 2; methionyl aminopeptidase 2; MNPEP; p67; p67EC 3.4.11.18; p67eIF2; Peptidase M 2

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Citations for Human Methionine Aminopeptidase 2/
METAP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation
    Authors: EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin
    Cell Reports, 2023-01-16;0(0):111965.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
    Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
    Nature Chemical Biology, 2022-02-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Pig epiblast stem cells depend on activin/nodal signaling for pluripotency and self-renewal.
    Authors: Alberio R, Croxall N, Allegrucci C
    Stem Cells Dev., 2010-10-01;19(10):1627-36.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC

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METAP2 Antibody

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