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Human MGMT Antibody

Catalog #: AF3794
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3794
AF3794-SP
Detection of Human MGMT by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MGMT Antibody Summary

Species Reactivity
Human
Specificity
Detects human MGMT in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MGMT
Met1-Asn207
Accession # P16455
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MGMT antibody by Western Blot. View Larger

Detection of Human MGMT by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human MGMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3794) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MGMT at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MGMT

The O-6-methylguanine-DNA-methyl transferase (MGMT) is involved in the direct repair of DNA alkylated at the O(6) position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation.

Long Name
O-6 Methylguanine-DNA Methyltransferase
Entrez Gene IDs
4255 (Human); 17314 (Mouse)
Alternate Names
6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase

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Citations for Human MGMT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expression of O<sup>6</sup>-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue
    Authors: Kimiko Ishiguro, Krishnamurthy Shyam, Philip G. Penketh, Raymond P. Baumann, Alan C. Sartorelli, Thomas J. Rutherford et al.
    Journal of Cancer Therapy
  2. BET protein inhibition sensitizes glioblastoma cells to temozolomide treatment by attenuating MGMT expression
    Authors: A Tancredi, O Gusyatiner, P Bady, MC Buri, R Lomazzi, D Chiesi, M Messerer, ME Hegi
    Cell Death & Disease, 2022-12-13;13(12):1037.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB
  3. Repair and translesion synthesis of O6-alkylguanine DNA lesions in human cells
    Authors: H Du, P Wang, Y Wang
    J. Biol. Chem., 2019-06-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. p53 Small-molecule inhibitor enhances temozolomide cytotoxic activity against intracranial glioblastoma xenografts.
    Authors: Dinca EB, Lu KV, Sarkaria JN, Pieper RO, Prados MD, Haas-Kogan DA, Vandenberg SR, Berger MS, James CD
    Cancer Res., 2008-12-15;68(24):10034-9.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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