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Human MIF Biotinylated Antibody

Catalog #: BAF289
14 Citations Datasheet / COA / SDS
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BAF289
Detection of MIF in HL-60 Human Cell Line by Flow Cytometry.
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Citations (14)
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Human MIF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse MIF in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MIF (R&D Systems, Catalog # 289-MF)
Pro2-Ala115
Accession # AAA36315
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MIF (Catalog # 289-MF)
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
HL‑60 human acute promyelocytic leukemia cell line treated with LPS, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of MIF in HL-60 Human Cell Line by Flow Cytometry. HL-60 human promyelocytic leukemia cell line was treated with 1 μg/ml LPS overnight then stained with Biotinylated Goat Anti-Human MIF Polyclonal Antibody (Catalog # BAF289, filled histogram) or Biotinylated Goat IgG control antibody (BAF108, open histogram), followed by Phycoerythrin-conjugated Streptavidin (F0040). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIF

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.

References
  1. Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
  2. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
  3. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
  4. Kozak, C.A. et al. (1995) Genomics 27:405.
  5. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
  6. Paralkar, V. and G. Wistow (1994) Genomics 19:48.
  7. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
  8. Flieger, O. et al. (2003) FEBS Lett. 551:78.
  9. Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
  10. Sun, H-W. et al. (1996) Protein Eng. 9:631.
  11. Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
  12. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
  13. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
  14. Bernhagen, J. et al. (1994) Biochemistry 33:14144.
  15. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  16. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
Long Name
Macrophage Migration Inhibitory Factor
Entrez Gene IDs
4282 (Human); 17319 (Mouse)
Alternate Names
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase

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Citations for Human MIF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. What Is the Significance of Perioperative Release of Macrophage Migration Inhibitory Factor in Cardiac Surgery?
    Authors: Christian Stoppe, Thilo Werker, Rolf Rossaint, Florian Dollo, Hongqi Lue, Willibald Wonisch et al.
    Antioxidants & Redox Signaling
  2. Hypoxia-induced endothelial secretion of macrophage migration inhibitory factor and role in endothelial progenitor cell recruitment
    Authors: David Simons, Gerrit Grieb, Mihail Hristov, Norbert Pallua, Christian Weber, Jürgen Bernhagen et al.
    Journal of Cellular and Molecular Medicine
  3. Role of the Macrophage Migration Inhibitory Factor (MIF) in the survival of first trimester human placenta under induced stress conditions
    Authors: F Ietta, EAV Ferro, E Bevilacqua, L Benincasa, E Maioli, L Paulesu
    Sci Rep, 2018-08-14;8(1):12150.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016-07-25;6(0):29909.
    Species: Human
    Sample Types: Serum
  5. Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
    Authors: Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N
    PLoS ONE, 2015-09-08;10(9):e0137366.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Detection)
  6. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  7. Effects of dexamethasone on macrophage migration inhibitory factor production in sepsis.
    Authors: Bruhn A, Verdant C, Vercruysse V, Su F, Vray B, Vincent JL
    Shock, 2006-08-01;26(2):169-73.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  8. Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis.
    Authors: Matsumoto K, Maruyama N, Maruyama T, Ohnishi Y, Nonaka S, Inoshita A, Ito K, Kitajima S, Abe M, Satomura A, Fujita T
    Clin. Exp. Immunol., 2005-02-01;139(2):338-47.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development
  9. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor.
    Authors: Ashcroft GS, Mills SJ, Lei K, Gibbons L, Jeong MJ, Taniguchi M, Burow M, Horan MA, Wahl SM, Nakayama T
    J. Clin. Invest., 2003-05-01;111(9):1309-18.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy.
    Authors: Matsumoto K, 2019, Kanmatsuse K
    7587, 2002-10-01;92(2):309-15.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development
  11. Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
    Authors: Wang L, Das H, Kamath A, Li L, Bukowski JF
    J. Immunol., 2002-05-15;168(10):4889-96.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Xenon triggers pro-inflammatory effects and suppresses the anti-inflammatory response compared to sevoflurane in patients undergoing cardiac surgery
    Authors: Thomas Breuer, Christoph Emontzpohl, Mark Coburn, Carina Benstoem, Rolf Rossaint, Gernot Marx et al.
    Critical Care
  13. Interaction of MIF Family Proteins in Myocardial Ischemia/Reperfusion Damage and Their Influence on Clinical Outcome of Cardiac Surgery Patients
    Authors: Christian Stoppe, Steffen Rex, Andreas Goetzenich, Sandra Kraemer, Christoph Emontzpohl, Josefin Soppert et al.
    Antioxidants & Redox Signaling
  14. Differential roles of angiogenic chemokines in endothelial progenitor cell-induced angiogenesis
    Authors: Isabella Kanzler, Nancy Tuchscheerer, Guy Steffens, Sakine Simsekyilmaz, Simone Konschalla, Andreas Kroh et al.
    Basic Research in Cardiology

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