Human Mind Bomb 2/MIB2 Antibody

Catalog #: MAB7289 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7289
MAB7289-SP

Mind Bomb 2/MIB2 in U266 Human Cell Line.

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Human Mind Bomb 2/MIB2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mind Bomb 2/MIB2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 739501

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Mind Bomb 2/MIB2 in U266 Human Cell Line. Mind Bomb 2/MIB2 was detected in immersion fixed U266 human myeloma cell line using Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB7289) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.