Human Mind Bomb 2/MIB2 Antibody

Catalog #: MAB72891 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB72891
MAB72891-SP

Detection of Human Mind Bomb 2/MIB2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mind Bomb 2/MIB2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 739505

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Mind Bomb 2/MIB2 by Western Blot. Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB72891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mind Bomb 2/MIB2 at approximately 103-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mind Bomb 2/MIB2

MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.

Long Name

Mindbomb Homolog 2

Entrez Gene IDs

142678 (Human); 76580 (Mouse)

Alternate Names

E3 ubiquitin-protein ligase MIB2; EC 6.3.2; EC 6.3.2.-; EC 6.3.2.13; FLJ20648; FLJ41406; MIB2; Mind Bomb 2; Mind bomb homolog 2; Mind Bomb-2; mindbomb homolog 2 (Drosophila); Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; SKD; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1; ZZ type with ankyrin repeat domain 1; ZZANK1; ZZZ5