Human Mindin Antibody

Catalog #: MAB2609 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2609
MAB2609-SP

All Mindin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mindin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mindin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 331620

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mindin
Glu32-Val331
Accession # AAH36341

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Mindin, under non-reducing conditions only.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mindin

Mindin, also known as Spondin-2 and DIL-1 (Differentially expressed in cancerous and non-cancerous lung cells 1) belongs to the F-spondin family of secreted extracellular matrix proteins. Family members are characterized by two N-terminal F-spondin domains (FS1 and FS2), and a third C-terminal thrombospondin type I repeat (TSP-1). Secreted Mindin exists as a disulfide-linked dimer that also forms higher order oligomers. It functions as a pattern recognition receptor for microbes and an adhesion molecule for neurons. Mindin is secreted by neurons, macrophages and fibroblasts in a variety of tissues. Human Mindin shares 88% amino acid sequence homology with mouse and rat proteins.

Entrez Gene IDs

10417 (Human); 100689 (Mouse)

Alternate Names

Differentially expressed in cancerous and non-cancerous lung cells 1; DIL1; DIL-1; DIL1FLJ34460; DKFZp686G21139; FLJ16313; FLJ22401; Mindin; M-SPONDIN; RG-1; SPON2; Spondin 2; spondin 2, extracellular matrix protein; spondin-2