Human MKP-3 Antibody

Catalog #: MAB35761 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB35761-100
MAB35761-SP

MKP‑3 in PC-3 and K562 Human Cell Lines.

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All MKP-3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MKP-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MKP-3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 363209

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MKP-3
Met1-Thr381
Accession # Q16828

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MKP‑3 in PC-3 and K562 Human Cell Lines. MKP-3 was detected in immersion fixed PC-3 human prostate cancer cell line (left panel; positive stain) and K562 human chronic myelogenous leukemia cell line (right panel; negative stain) using Mouse Anti-Human MKP-3 Monoclonal Antibody (Catalog # MAB35761) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKP-3

MAP Kinase Phosphatase 3 (MKP-3), also called PYST1 and Dual Specificity Phosphatase 6 (DUSP6), dephosphorylates serine, threonine, and tyrosine residues in proteins. On Western blots, MKP-3 has an apparent molecular weight of 42 kilodaltons. It has a high degree of substrate selectivity for dephosphorylating ERK kinases over stress-related kinases such as p38 and JNK. MKP-3 is ubiquitinylated prior to degradation in the proteosome. The rate of MKP-3 degradation is enhanced by ERK-mediated phosphorylation.

Long Name

MAP Kinase Phosphatase 3

Entrez Gene IDs

1848 (Human)

Alternate Names

dual specificity phosphatase 6; Dual specificity protein phosphatase PYST1; DUSP6; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP3; MKP-3; MKP3serine/threonine specific protein phosphatase; PYST1; PYST1dual specificity protein phosphatase 6