Human MLK2 Antibody

Catalog #: AF5066
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5066
AF5066-SP

Detection of Human MLK2 by Western Blot.

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All MLK2 Antibodies

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Citations (2)

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Human MLK2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human MLK2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MLK2
His345-Arg495
Accession # Q02779

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MLK2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia, and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5066) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK2 at approximately 104 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MLK2

MLK2 (mixed lineage kinase 2; also MST and MAP3K10) is a member of the STE Ser/Thr kinase family of enzymes. It is expressed in neurons, lymphoid tissue, and skeletal muscle, where it participates in apoptosis. Human MLK2 is 954 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 23‑76), followed by a kinase domain that likely acts to phosphorylate Ser/Thr (aa 99‑355), two Leu-zipper segments that mediate homodimerization (aa 384‑419) and a C-terminal Ser/Thr/Pro-rich region that undergoes phosphorylation at multiple sites (aa 497‑954). There is one splice variant that shows an in-frame 16 aa substitution for the 16 aa between aa 465‑480.

Long Name

Mixed Lineage Kinase 2

Entrez Gene IDs

4294 (Human); 269881 (Mouse); 308463 (Rat)

Alternate Names

EC 2.7.11; MAP3K10; MEKK10; mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 kinase; MLK2; MLK2MKN28 derived nonreceptor_type serine/threonine kinase; MST; MSTEC 2.7.11.25; Protein kinase MST

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Product Datasheet

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Related Research Areas

Citations for Human MLK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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