Human/Mouse Bcl-6 Antibody Summary
Glu522-Thr696
Accession # Q8CB25
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse and Human Bcl‑6 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Bcl-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Detection of Human Bcl‑6 by Simple WesternTM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑6 at approximately 111 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Bcl‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-6
Bcl-6 (B-cell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is 706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32‑99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518‑681). Centrally located are three PEST sequences (aa 336‑430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6.
Product Datasheets
Citation for Human/Mouse Bcl-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
Authors: Kinchen J, Chen HH, Parikh K et al.
Cell
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse Bcl-6 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Bcl-6 Antibody and earn rewards!
Have you used Human/Mouse Bcl-6 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
