Human/Mouse BDNF DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human BDNF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: BDNF
BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Human/Mouse BDNF DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Controlled Hypoxia Acutely Prevents Physical Inactivity-Induced Peripheral BDNF Decline
Authors: Duderstadt, Y;Schreiber, S;Burtscher, J;Schega, L;Müller, NG;Brigadski, T;Braun-Dullaeus, RC;Le beta mann, V;Müller, P;
International journal of molecular sciences
Species: Human
Sample Types: Serum, Plasma
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Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders
Authors: Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P;
Psychoneuroendocrinology
Species: Human
Sample Types: Plasma
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BDNF-loaded PDADMAC-heparin multilayers: a novel approach for neuroblastoma cell study
Authors: D?bkowska, M;Stukan, I;Kowalski, B;Donerowicz, W;Wasilewska, M;Szatanik, A;Sta?czyk-Dunaj, M;Michna, A;
Scientific reports
Species: Human
Sample Types: Cell Culture Supernates
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Chronic hyperpalatable diet induces impairment of hippocampal-dependent memories and alters glutamatergic and fractalkine axis signaling
Authors: Ribeiro, R;Silva, EG;Moreira, FC;Gomes, GF;Cussat, GR;Silva, BSR;da Silva, MCM;de Barros Fernandes, H;de Sena Oliveira, C;de Oliveira Guarnieri, L;Lopes, V;Ferreira, CN;de Faria, AMC;Maioli, TU;Ribeiro, FM;de Miranda, AS;Moraes, GSP;de Oliveira, ACP;Vieira, LB;
Scientific reports
Species: Mouse
Sample Types: Serum, Tissue Homogenates
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Changes in Adipokine, Resitin, and BDNF Concentrations in Treatment-Resistant Depression after Electroconvulsive Therapy
Authors: Permoda-Pachuta, A;Malewska-Kasprzak, M;Skibi?ska, M;Rzepski, K;Dmitrzak-W?glarz, M;
Brain sciences
Species: Human
Sample Types: Serum
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Cefaclor causes vagus nerve-mediated depression-like symptoms with gut dysbiosis in mice
Authors: Joo, MK;Shin, YJ;Kim, DH;
Scientific reports
Species: Mouse
Sample Types: Tissue Homogenates
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The relationship between the brain-derived neurotrophic factor and neurocognitive response to physical exercise in individuals with schizophrenia
Authors: Bang-Kittilsen, G;Egeland, J;Ueland, T;Andersen, E;Bigseth, TT;Holmen, TL;Mordal, J;Holst, R;Engh, JA;
Psychoneuroendocrinology
Species: Human
Sample Types: Platelet-Poor Plasma
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Inflammatory biomarkers at different stages of Sarcopenia in older women
Authors: da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR;
Scientific reports
Species: Human
Sample Types: Plasma
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Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease
Authors: Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
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Omega-3 polyunsaturated fatty acids (n-3 PUFAs), somatic and fatigue symptoms in cardiovascular diseases comorbid major depressive disorder (MDD): A randomized controlled trial
Authors: Chang, JP;Chang, SS;Chen, HT;Chien, YC;Yang, HT;Huang, SY;Tseng, PT;Chang, CH;Galecki, P;Su, KP;
Brain, behavior, and immunity
Species: Human
Sample Types: Plasma
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Composite Coatings Based on Recombinant Spidroins and Peptides with Motifs of the Extracellular Matrix Proteins Enhance Neuronal Differentiation of Neural Precursor Cells Derived from Human Induced Pluripotent Stem Cells
Authors: EV Novosadova, OV Dolotov, LV Novosadova, LI Davydova, KV Sidoruk, EL Arsenyeva, DM Shimchenko, VG Debabov, VG Bogush, VZ Tarantul
International Journal of Molecular Sciences, 2023-03-02;24(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Effects of Exercise Training on Neurotrophic Factors and Blood-Brain Barrier Permeability in Young-Old and Old-Old Women
Authors: SY Cho, HT Roh
International Journal of Environmental Research and Public Health, 2022-12-16;19(24):.
Species: Human
Sample Types: Serum
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Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study
Authors: LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues
Journal of Clinical Medicine, 2022-12-02;11(23):.
Species: Human
Sample Types: Plasma
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Chronic Methylmercury Intoxication Induces Systemic Inflammation, Behavioral, and Hippocampal Amino Acid Changes in C57BL6J Adult Mice
Authors: TS Nascimento, DV Pinto, RP Dias, RS Raposo, PIG Nunes, CR Roque, FA Santos, GM Andrade, JL Viana, AH Fostier, A Sussulini, JI Alvarez-Le, C Fontes-Rib, JO Malva, RB Oriá
International Journal of Molecular Sciences, 2022-11-10;23(22):.
Species: Mouse
Sample Types: Tissue Homogenates
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Circulating and Salivary NGF and BDNF Levels in SARS-CoV-2 Infection: Potential Predictor Biomarkers of COVID-19 Disease-Preliminary Data
Authors: F Biamonte, A Re, BO Balzamino, G Ciasca, D Santucci, C Napodano, G Nocca, A Fiorita, M Marino, U Basile, A Micera, CAM Callà
Journal of personalized medicine, 2022-11-09;12(11):.
Species: Human
Sample Types: Serum
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Gentle Touch Therapy, Pain Relief and Neuroplasticity at Baseline in Fibromyalgia Syndrome: A Randomized, Multicenter Trial with Six-Month Follow-Up
Authors: ASI Salgado, MH Takemoto, CFTC de Souza, DC Salm, D da Rosa, GC Cardoso, DD Ludtke, SFC Soares, JK Ferreira, AR Dutra, YC Szeremeta, G Mazzardo, J Mayra, DDL Sheffer, W Caumo, EB Bittencour, R Schleip, A Latini, F Bobinski, DF Martins
Journal of Clinical Medicine, 2022-08-20;11(16):.
Species: Human
Sample Types: Serum
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BDNF, proBDNF and IGF-1 serum levels in na�ve and medicated subjects with autism
Authors: MLA Robinson-A, B Michalski, B Vidal-Mart, LR Hernández, MW Santiesteb, M Fahnestock
Scientific Reports, 2022-08-12;12(1):13768.
Species: Human
Sample Types: Serum
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Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli
Authors: P Szpakowski, D Ksiazek-Wi, M Turniak-Ku, I Pacan, A Glabinski
Biomedicines, 2022-07-22;10(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Celastrol alleviates high-fat diet-induced obesity via enhanced muscle glucose utilization and mitochondrial oxidative metabolism-mediated upregulation of pyruvate dehydrogenase complex
Authors: MHA Bakar, NSN Shahril, MSFM Khalid, S Mohammad, KA Shariff, T Karunakara, RM Salleh, MN Rosdi
Oncogene, 2022-06-06;449(0):116099.
Species: Mouse
Sample Types: Serum
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DHA-Enriched Fish Oil Ameliorates Deficits in Cognition Associated with Menopause and the APOE4 Genotype in Rodents
Authors: MG Pontifex, A Martinsen, RNM Saleh, G Harden, C Fox, M Muller, D Vauzour, AM Minihane
Nutrients, 2022-04-19;14(9):.
Species: Mouse
Sample Types: Serum
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ProBDNF as an Indicator of Improvement among Women with Depressive Episodes
Authors: W Zwoli?ska, M Skibinska, A S?opie?, M Dmitrzak-W
Metabolites, 2022-04-16;12(4):.
Species: Human
Sample Types: Serum
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Endothelial Unc5B controls blood-brain barrier integrity
Authors: K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann
Nature Communications, 2022-03-04;13(1):1169.
Species: Mouse
Sample Types: EDTA Plasma
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Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula
Authors: S Hakki, EJ Robinson, MG Robson
Scientific Reports, 2022-01-26;12(1):1427.
Species: Human
Sample Types: Plasma
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Comparison of the effects of open vs. closed skill exercise on the acute and chronic BDNF, IGF-1 and IL-6 response in older healthy adults
Authors: T Behrendt, F Kirschnick, L Kröger, P Beileke, M Rezepin, T Brigadski, V Le beta mann, L Schega
Bmc Neuroscience, 2021-11-25;22(1):71.
Species: Human
Sample Types: Plasma
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Apathy is not associated with a panel of biomarkers in older adults with HIV disease
Authors: SP Woods, JL Thompson, MA Babicz, L Shahani, GD Colpo, NP Rocha, EE Morgan, AL Teixeira
Journal of psychosomatic research, 2021-11-08;152(0):110666.
Species: Human
Sample Types: Plasma
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The Restorative Effect of Human Amniotic Fluid Stem Cells on Spinal Cord Injury
Authors: M Lale Ataei, M Karimipour, P Shahabi, R Pashaei-As, E Ebrahimie, M Pashaiasl
Cells, 2021-09-28;10(10):.
Species: Rat
Sample Types: Cell Culture Supernates
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NGF Eye Administration Recovers the TrkB and Glutamate/GABA Marker Deficit in the Adult Visual Cortex Following Optic Nerve Crush
Authors: P Rosso, E Fico, LA Mesentier-, V Triaca, A Lambiase, P Rama, P Tirassa
International Journal of Molecular Sciences, 2021-09-16;22(18):.
Species: Rat
Sample Types: Tissue Homogenates
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Inhibition of CSF1R, a receptor involved in microglia viability, alters behavioral and molecular changes induced by cocaine
Authors: MCM da Silva, GF Gomes, H de Barros, AM da Silva, AL Teixeira, FA Moreira, AS de Miranda, ACP de Oliveir
Scientific Reports, 2021-08-06;11(1):15989.
Species: Mouse
Sample Types: Tissue Homogenates
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C-terminal domain small phosphatase 1 (CTDSP1) regulates growth factor expression and axonal regeneration in peripheral nerve tissue
Authors: NM Gervasi, A Dimtchev, DM Clark, M Dingle, AV Pisarchik, LJ Nesti
Scientific Reports, 2021-07-14;11(1):14462.
Species: Human
Sample Types: Cell Culture Supernates
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A Single Session of Whole-Body Electromyostimulation Increases Muscle Strength, Endurance and proNGF in Early Parkinson Patients
Authors: G Fiorilli, F Quinzi, A Buonsenso, G Casazza, L Manni, A Parisi, A Di Costanz, G Calcagno, M Soligo, A di Cagno
International Journal of Environmental Research and Public Health, 2021-05-20;18(10):.
Species: Human
Sample Types: Cell Culture Supernates
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VEGF Mediates Retinal M�ller Cell Viability and Neuroprotection through BDNF in Diabetes
Authors: YZ Le, B Xu, AJ Chucair-El, H Zhang, M Zhu
Biomolecules, 2021-05-10;11(5):.
Species: Rat
Sample Types: Cell Culture Supernates
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Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
Authors: HL Lee, CE Yeum, H Lee, J Oh, JT Kim, WJ Lee, Y Ha, YI Yang, KN Kim
International Journal of Molecular Sciences, 2021-04-16;22(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Influence of Previous COVID-19 and Mastitis Infections on the Secretion of Brain-Derived Neurotrophic Factor and Nerve Growth Factor in Human Milk
Authors: V Demers-Mat, DJ Hines, RM Hines, S Lavangnana, S Fels, E Medo
International Journal of Molecular Sciences, 2021-04-08;22(8):.
Species: Human
Sample Types: Milk Supernates
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Evaluation of the Effect of Hypericum triquetrifolium Turra on Memory Impairment Induced by Chronic Psychosocial Stress in Rats: Role of BDNF
Authors: KH Alzoubi, L Abdel-Hafi, OF Khabour, T El-Elimat, MA Alzubi, FQ Alali
Drug Design, Development and Therapy, 2020-12-01;14(0):5299-5314.
Species: Rat
Sample Types: Tissue Homogenates
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The Effect of Long-Term Aroniamelanocarpa Extract Supplementation on Cognitive Performance, Mood, and Vascular Function: A Randomized Controlled Trial in Healthy, Middle-Aged Individuals
Authors: S Ahles, YR Stevens, PJ Joris, D Vauzour, J Adam, E de Groot, J Plat
Nutrients, 2020-08-17;12(8):.
Species: Human
Sample Types: Serum
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Effects of Regular Taekwondo Intervention on Oxidative Stress Biomarkers and Myokines in Overweight and Obese Adolescents
Authors: HT Roh, SY Cho, WY So
Int J Environ Res Public Health, 2020-04-06;17(7):.
Species: Human
Sample Types: Plasma
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Neuroinflammatory Markers in the Serum of Prepubertal Children with Down Syndrome
Authors: L Tarani, V Carito, G Ferraguti, C Petrella, A Greco, M Ralli, MP Messina, D Rasio, E De Luca, C Putotto, P Versacci, M Ceccanti, M Fiore
J Immunol Res, 2020-03-23;2020(0):6937154.
Species: Human
Sample Types: Serum
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A Cross-Sectional Study Evaluating the Effects of Resistance Exercise on Inflammation and Neurotrophic Factors in Elderly Women with Obesity
Authors: HT Roh, SY Cho, WY So
J Clin Med, 2020-03-20;9(3):.
Species: Human
Sample Types: Serum
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Mast Cells Populate the Corneoscleral Limbus: New Insights for Our Understanding of Limbal Microenvironment
Authors: A Micera, K Jirsova, G Esposito, BO Balzamino, A Di Zazzo, S Bonini
Invest. Ophthalmol. Vis. Sci., 2020-03-09;61(3):43.
Species: Human
Sample Types: Cell Culture Supernates
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The importance of physical fitness for the relationship of BDNF with obesity measures in young normal-weight adults
Authors: MA Alomari, OF Khabour, K Alawneh, KH Alzoubi, AB Maikano
Heliyon, 2020-03-03;6(3):e03490.
Species: Human
Sample Types: Serum
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Chronic Systemic Inflammation Exacerbates Neurotoxicity in a Parkinson's Disease Model
Authors: P Ugalde-Muñ, I Fetter-Pru, L Navarro, E García, A Chavarría
Oxid Med Cell Longev, 2020-01-13;2020(0):4807179.
Species: Mouse
Sample Types: Cell Culture Supernates
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The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates
Authors: O Nebie, D Devos, V Vingtdeux, L Barro, JC Devedjian, A Jonneaux, ML Chou, R Bordet, L Buée, F Knutson, D Blum, T Burnouf
J. Biomed. Sci., 2019-10-31;26(1):89.
Species: Human
Sample Types: Cell Culture Supernates
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Greater Circulating Copper Concentrations and Copper/Zinc Ratios are Associated with Lower Psychological Distress, But Not Cognitive Performance, in a Sample of Australian Older Adults
Authors: M Mravunac, EA Szymlek-Ga, R M Daly, B R Roberts, M Formica, J Gianoudis, S L O'Connel, C A Nowson, B R Cardoso
Nutrients, 2019-10-17;11(10):.
Species: Human
Sample Types: Serum
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Associations between inflammatory and neurological markers with quality of life and well-being in older adults
Authors: JL Tait, RL Duckham, CM Milte, LC Main, RM Daly
Exp. Gerontol., 2019-07-16;125(0):110662.
Species: Human
Sample Types: Serum
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PER3 variable number tandem repeat (VNTR) polymorphism modulates the circadian variation of the descending pain modulatory system in healthy subjects
Authors: F Carvalho, M Pedrazzoli, A Gasparin, F Dos Santos, M Zortea, A Souza, I da Silva L, F Fregni, W Caumo
Sci Rep, 2019-06-27;9(1):9363.
Species: Human
Sample Types: Serum
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Cerebrospinal fluid BDNF pro-peptide levels in major depressive disorder and schizophrenia
Authors: T Mizui, K Hattori, S Ishiwata, S Hidese, S Yoshida, H Kunugi, M Kojima
J Psychiatr Res, 2019-03-26;113(0):190-198.
Species: Mouse
Sample Types: CSF
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Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
Int J Mol Sci, 2018-11-28;19(12):.
Species: Rat
Sample Types: Hydrogel Implant
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Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells
Authors: MS Chen, TJ Wang, HC Lin, B Thierry
New biotechnology, 2018-11-20;0(0):.
Species: Human
Sample Types: Platelet Lysates
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Dance training is superior to repetitive physical exercise in inducing brain plasticity in the elderly
Authors: K Rehfeld, A Lüders, A Hökelmann, V Lessmann, J Kaufmann, T Brigadski, P Müller, NG Müller
PLoS ONE, 2018-07-11;13(7):e0196636.
Species: Human
Sample Types: Serum
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Lifelong environmental enrichment in the absence of exercise protects the brain from age-related cognitive decline
Authors: AM Birch, ÁM Kelly
Neuropharmacology, 2018-04-07;0(0):.
Species: Rat
Sample Types: Tissue Culture Supernates
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Does Transcranial Direct Current Stimulation Combined with Peripheral Electrical Stimulation Have an Additive Effect in the Control of Hip Joint Osteonecrosis Pain Associated with Sickle Cell Disease? A Protocol for a One-Session Double Blind, Block-Randomized Clinical Trial
Authors: TDS Lopes, WDS Silva, SB Ribeiro, CA Figueiredo, FQ Campbell, GC Daltro, A Valenzuela, P Montoya, RCS Lucena, AF Baptista
Front Hum Neurosci, 2017-12-20;11(0):633.
Species: Human
Sample Types: Serum
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Association between serum levels of glutamate and neurotrophic factors and response to clozapine treatment
Authors: A Krivoy, E Hochman, KV Sendt, S Hollander, Y Vilner, M Selakovic, A Weizman, M Taler
Schizophr. Res., 2017-06-07;0(0):.
Species: Human
Sample Types: Serum
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Effect of 2-octylcyanoacrylate on placenta derived mesenchymal stromal cells on extracellular matrix
Authors: YJ Chen, L Lankford, S Kabagambe, Z Saenz, P Kumar, D Farmer, A Wang
Placenta, 2017-04-02;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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High Intensity Exercise as a Dishabituating Stimulus Restores Counterregulatory Responses in Recurrently Hypoglycemic Rodents
Authors: AD McNeilly, JR Gallagher, JT Huang, ML Ashford, RJ McCrimmon
Diabetes, 2017-03-07;0(0):.
Species: Rat
Sample Types: Whole Blood
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Effects of Ranibizumab and Aflibercept on Human M�ller Cells and Photoreceptors under Stress Conditions
Authors: W Shen, B Yau, SR Lee, L Zhu, M Yam, MC Gillies
Int J Mol Sci, 2017-03-01;18(3):.
Species: Human
Sample Types: Cell Culture Supernates
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Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
PLoS ONE, 2017-01-09;12(1):e0168565.
Species: Human
Sample Types: Serum
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Serum levels of neurotrophic factors in active toxoplasmic retinochoroiditis
Authors: Cynthia Azeredo Cordeiro
Braz J Infect Dis, 2016-12-05;0(0):.
Species: Human
Sample Types: Serum
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Neuroendocrine Inflammatory Responses in Overweight/Obese Infants
PLoS ONE, 2016-12-01;11(12):e0167593.
Species: Human
Sample Types: Plasma
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Pharmacologically active microcarriers delivering BDNF within a hydrogel: novel strategy for human bone marrow-derived stem cells neural/neuronal differentiation guidance and therapeutic secretome enhancement
Authors: Claudia N Montero-Me
Acta Biomater, 2016-11-16;0(0):.
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Chrysin promotes attenuation of depressive-like behavior and hippocampal dysfunction resulting from olfactory bulbectomy in mice
Authors: Silvana Peterini Boeira
Chem. Biol. Interact., 2016-11-03;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Myokines in Response to a Tournament Season among Young Tennis Players
Biomed Res Int, 2016-08-29;2016(0):1460892.
Species: Human
Sample Types: Serum
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Transplantation of human adipose tissue-derived stem cells delays clinical onset and prolongs life span in ALS mouse model.
Authors: Kim, Kwang S, Lee, Hong J, An, Jin, Kim, Yun B, Ra, Jung Cha, Lim, Inja, Kim, Seung U
Cell Transplant, 2013-09-18;23(12):1585-97.
Species: Human
Sample Types: Cell Culture Supernates
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Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
Authors: Manley, Nathan C, Caso, Javier R, Works, Melissa, Cutler, Andrew B, Zemlyak, Ilona, Sun, Guohua, Munhoz, Carolina, Chang, Sydney, Sorrells, Shawn F, Ermini, Florian, Decker, Johannes, Bertrand, Anthony, Dinkel, Klaus M, Steinberg, Gary K, Sapolsky, Robert M
PLoS ONE, 2013-04-16;8(4):e61789.
Species: Human
Sample Types: Cell Culture Supernates
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Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
Brain, 2010-07-21;133(0):2232-47.
Species: Human
Sample Types: Cell Culture Supernates
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Serum levels of brain-derived neurotrophic factor correlate with motor impairment in Parkinson's disease.
Authors: Scalzo P, Kummer A, Bretas TL, Cardoso F, Teixeira AL
J. Neurol., 2009-10-22;257(4):540-5.
Species: Human
Sample Types: Serum
-
Helminth infections associated with multiple sclerosis induce regulatory B cells.
Authors: Correale J, Farez M, Razzitte G
Ann. Neurol., 2008-08-01;64(2):187-99.
Species: Human
Sample Types: Cell Culture Supernates
-
Increased plasma brain-derived neurotrophic factor levels in chronic smokers following unaided smoking cessation.
Authors: Kim TS, Kim DJ, Lee H, Kim YK
Neurosci. Lett., 2007-07-04;423(1):53-7.
Species: Human
Sample Types: Plasma
-
Decreased plasma BDNF level in depressive patients.
Authors: Lee BH, Kim H, Park SH, Kim YK
J Affect Disord, 2006-12-13;101(1):239-44.
Species: Human
Sample Types: Plasma
-
Relationship of serum brain-derived neurotrophic factor level with other markers of disease severity in patients with atopic dermatitis.
Authors: Namura K, Hasegawa G, Egawa M, Matsumoto T, Kobayashi R, Yano T, Katoh N, Kishimoto S, Ohta M, Obayashi H, Ose H, Fukui M, Nakamura N, Yoshikawa T
Clin. Immunol., 2006-12-11;122(2):181-6.
Species: Human
Sample Types: Serum
-
Circulating levels of brain-derived neurotrophic factor correlate with disease severity in the intrinsic type of atopic dermatitis.
Authors: Raap U, Werfel T, Goltz C, Deneka N, Langer K, Bruder M, Kapp A, Schmid-Ott G, Wedi B
Allergy, 2006-12-01;61(12):1416-8.
Species: Human
Sample Types: Serum
-
Selected neurotrophins, neuropeptides, and cytokines: developmental trajectory and concentrations in neonatal blood of children with autism or Down syndrome.
Authors: Nelson PG, Kuddo T, Song EY
Int. J. Dev. Neurosci., 2005-11-14;24(1):73-80.
Species: Human
Sample Types: Complex Sample Type
-
Neurotrophic factors in relapsing remitting and secondary progressive multiple sclerosis patients during interferon beta therapy.
Authors: Caggiula M, Batocchi AP, Frisullo G, Angelucci F, Patanella AK, Sancricca C, Nociti V, Tonali PA, Mirabella M
Clin. Immunol., 2005-11-07;118(1):77-82.
Species: Human
Sample Types: Cell Culture Supernates
-
Maternal poly I:C exposure during pregnancy regulates TNF alpha, BDNF, and NGF expression in neonatal brain and the maternal-fetal unit of the rat.
Authors: Gilmore JH, Jarskog LF, Vadlamudi S
J. Neuroimmunol., 2004-11-24;159(1):106-12.
Species: Rat
Sample Types: Tissue Homogenates
-
Intracerebral transplantation of carotid body in rats with transient middle cerebral artery occlusion.
Authors: Yu G, Xu L, Hadman M, Hess DC, Borlongan CV
Brain Res., 2004-07-23;1015(1):50-6.
Species: Rat
Sample Types: Tissue Homogenates
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