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Human/Mouse CD44 Antibody

Catalog #: MAB6127
7 Citations 1 Review Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB6127
MAB6127-SP
Detection of CD44 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CD44 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CD44 in flow cytometry.
Source
Recombinant Monoclonal Rat IgG Clone # IM7.8.1R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Myeloid leukemia M1 cells induced with Dexamethasone
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Flow Cytometry Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44, also known as ECMR-III, Hermes, Pgp1, Ly-24, GP90, or HUTCH, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44.

Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Citations for Human/Mouse CD44 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following beta ‑catenin silencing
    Authors: Xidan Zhu, Jia Feng, Wenguang Fu, Xiaojia Shu, Xue Wan, Jinbo Liu
    International Journal of Molecular Medicine
  2. siRNA-encapsulated immunoliposomes conjugated with CD44 antibodies target and eliminate gastric cancer-initiating cells
    Authors: F Yang, Z Zheng, L Zheng, J Qin, H Li, X Xue, J Gao, G Fang
    Onco Targets Ther, 2018-10-11;11(0):6811-6825.
    Species: Human
    Sample Types: Whole Cells
    Applications: Complex Application
  3. Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity
    Authors: KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona
    Cancer Immunol Res, 2017-11-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Nicotinamide metabolism regulates glioblastoma stem cell maintenance
    Authors: J Jung, LJY Kim, X Wang, Q Wu, T Sanvoranar, CG Hubert, BC Prager, LC Wallace, X Jin, SC Mack, JN Rich
    JCI Insight, 2017-05-18;2(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016-10-20;2016(0):6104780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Crucial role of hyaluronan in neointimal formation after vascular injury.
    Authors: Kashima Y, Takahashi M, Shiba Y, Itano N, Izawa A, Koyama J, Nakayama J, Taniguchi S, Kimata K, Ikeda U
    PLoS ONE, 2013-03-06;8(3):e58760.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Enhanced targeting of prostate cancer‑initiating cells by salinomycin‑encapsulated lipid‑PLGA nanoparticles linked with CD44 antibodies
    Authors: Jun Wei, Jin Sun, Yu Liu
    Oncology Letters

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Human/Mouse CD44 Antibody
By Tina Yuan on 08/06/2023
Application: IHC Sample Tested: epithelium Species: Mouse
Immunohistochemistry Human/Mouse CD44 Antibody MAB6127