Human/Mouse CDC73 Antibody

Catalog #: AF5508 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5508
AF5508-SP

Detection of Human and Mouse CDC73 by Western Blot.

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All CDC73 Antibodies

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Human/Mouse CDC73 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse CDC73 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CDC73
Met1-Glu260
Accession # Q6P1J9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse CDC73 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDC73 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for CDC73 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDC73

CDC73 (also Parafibromin, HRPT2, and C1orf28) is a 60‑65 kDa member of the Cell Division Cycle family of proteins. It is found in adrenal, parathyroid and kidney, and serves as a regulator of Wnt signaling and a tumor suppressor by blocking c-myc expression. Human CDC73 is 531 amino acids (aa) in length. It contains multiple NLSs (aa 125‑139, 76‑92, and 393‑410) and a beta -catenin interaction sequence (aa 218‑263). There are at least two potential splice variants. One shows a five Lys substitution for aa 300‑531, while a second shows a 19 aa substitution for aa 298‑531. Full-length human and mouse CDC73 are completely (100%) identical in primary aa sequence.

Long Name

Cell Division Cycle 73

Entrez Gene IDs

79577 (Human); 214498 (Mouse); 304832 (Rat)

Alternate Names

C1orf28hyperparathyroidism 2 (with jaw tumor); CDC73; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S.cerevisiae); Cell division cycle protein 73 homolog; chromosome 1 open reading frame 28; HPTJT; HPT-JT; HRPT2; HRPT2FLJ23316; Hyperparathyroidism 2 protein; HYX; Parafibromin