Human/Mouse Cyclin A2 Antibody Summary
Ala73-Tyr199
Accession # P20248
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Cyclin A2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin A2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin A2
Cyclin A2 (also CCNA2) is a member of the cyclin AB subfamily, cyclin family of proteins. Cyclin A2 is ubiquitously expressed, in contrast to CyclinA1 that only appears in late pachytene spermatocytes. It associates with CDK1 during G2 and M phase, and CDK2 in S phase, of the cell cycle. Binding to SCAPER restricts Cyclin A2 to the cytoplasm. In both compartments, it provides substrate specificity to a phosphorylating complex that promotes entry into, and progression through, mitosis. Human Cyclin A2 is 432 amino acids (aa) in length. It contains two cyclin box folds (aa 210‑299 and 309‑430) and four acetylation sites that induce molecule turnover (Lys54/68/95/112). Over aa 73‑199, human Cyclin A2 shares 83% aa identity with mouse Cyclin A2.
Product Datasheets
Citations for Human/Mouse Cyclin A2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Cisplatin exposure causes c-Myc-dependent resistance to CDK4/6 inhibition in HPV-negative head and neck squamous cell carcinoma
Authors: AM Robinson, R Rathore, NJ Redlich, DR Adkins, T VanArsdale, BA Van Tine, LS Michel
Cell Death Dis, 2019-11-14;10(11):867.
Species: Xenograft
Sample Types: Tissue Homogenates
Applications: Western Blot -
Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
Haematologica, 2017-12-07;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
CREB is activated by muscle injury and promotes muscle regeneration.
Authors: Stewart R, Flechner L, Montminy M
PLoS ONE, 2011-09-13;6(9):e24714.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
J. Biol. Chem., 2011-05-14;286(27):23982-95.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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