Human/Mouse ELF3 Antibody

Catalog #: MAB5787 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5787
MAB5787-SP

Detection of Human and Mouse ELF3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse ELF3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse ELF3 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 662533

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ELF3
Met1-Gly173
Accession # P78545

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse ELF3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ELF3 Monoclonal Antibody (Catalog # MAB5787) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ELF3

ELF3 (E74-Like Factor 3), also known as ESE-1, ESX, ERT and JEN, is a 41-43 kDa member of the ETS family of proteins. During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation. Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2. Human ELF3 is 371 amino acids (aa) in length. It contains one PNT/pointed dimerization domain (aa 46-132), a protein stabilizing PEST sequence (aa 210-225), an A/T Hook region that binds to AT-rich DNA sequences (aa 236-252), and an ETS DNA binding domain (aa 273-355). ELF3 interacts with CREBBP, EP300, KU70 and KU86. There is one splice variant that shows a deletion of aa 174-200. Over aa 1-173, human ELF3 shares 87% aa identity with mouse ELF3.

Long Name

E74-like factor 3

Entrez Gene IDs

1999 (Human); 13710 (Mouse); 304815 (Rat)

Alternate Names

E74-like factor 3 (ets domain transcription factor, epithelial-specific ); E74-like factor 3; ELF3; Epithelial-restricted with serine box; epithelial-specific); Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; EPR1; EPR-1; ERT; ESX; ets domain transcription factor, serine box (epithelial-specific); ETS-related transcription factor Elf-3; JEN; serine box