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Human/Mouse ID1 Antibody

Catalog #: AF4377
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4377
AF4377-SP
Detection of Human ID1 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse ID1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ID1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse ID1
Met1-Glu135
Accession # P20067
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
HepG2 human hepatocellular carcinoma cell line
Immunocytochemistry
5-15 µg/mL
Immersion fixed BG01V human embryonic stem cells differentiated to neural progenitor cells, PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ID1 antibody by Western Blot. View Larger

Detection of Human ID1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ID1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

ID1 in BG01V Human Embryonic Stem Cells. ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western View Larger

Detection of Human ID1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ID1 at approximately 30 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry View Larger

Detection of ID1 in PC‑3 cells (Positive) & Daudi cells (Negative). ID1 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ID1

ID-1a is a negative regulator of helix-loop-helix (HLH) DNA binding proteins. ID-1a contains a HLH motif but no DNA binding motif, therefore, upon binding other HLH proteins, ID-1a acts a dominant negative regulator. ID-1a can be found in both cytoplasmic and nuclear cell fractions. Increased ID-1a expression is associated with cellular proliferation and cancer.

Long Name
Inhibitor of DNA Binding 1
Entrez Gene IDs
3397 (Human); 15901 (Mouse); 25261 (Rat)
Alternate Names
BHLHB24; bHLHb24DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein); dJ857M17.1.2; ID; ID1; inhibitor of differentiation 1; Inhibitor of DNA binding 1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

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Citations for Human/Mouse ID1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Suppression of ID1 expression in colon cancer cells increases sensitivity to 5-fluorouracil
    Authors: T Przyby?a, M Sakowicz-B, I Maciejewsk, H Bielarczyk, T Pawe?czyk
    Acta Biochim. Pol., 2017-05-17;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Characterization of BMS-911543, a functionally selective small-molecule inhibitor of JAK2.
    Authors: Purandare AV, McDevitt TM, Wan H
    Leukemia, 2011-10-21;26(2):280-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone
    Authors: Zhichao Zhang, Adeel Manaf, Yanjiao Li, Sonia Peña Perez, Rajikala Suganthan, John Arne Dahl et al.
    Molecular Neurobiology

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