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Human/Mouse ING1 Antibody

Catalog #: MAB5758
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5758
MAB5758-SP
Detection of Human and Mouse ING1 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse ING1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ING1 in Western blot.
Source
Monoclonal Mouse IgG1 Clone # 585915
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human ING1
Arg70-Ala184
Accession # Q9UK53
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse ING1 antibody by Western Blot. View Larger

Detection of Human and Mouse ING1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ING1 Monoclonal Antibody (Catalog # MAB5758) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ING1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ING1

ING1 (inhibitor of growth-1) is a member of the ING family of tumor suppressive and apoptosis-promoting epigenetic regulators of chromatin structure. They interact with and alter the ratio of pro- and anti-apoptotic Bcl-2 family members. The p53 tumor suppressor and its downstream targets can potentiate ING1-induced apoptosis. Human isoforms p33ING1, p24ING1c, p33ING1b and p47ING1a vary in N-terminal sequence, resulting in 279, 210, 235 and 422 aa proteins, respectively. The sequence used as an immunogen is common to all isoforms, and shares 83% and 81% amino acid (aa) identity with mouse and rat ING1, respectively.

Long Name
Inhibitor of Growth 1
Entrez Gene IDs
3621 (Human); 26356 (Mouse); 306626 (Rat)
Alternate Names
growth inhibitor ING1; growth inhibitory protein ING1; ING1; inhibitor of growth family, member 1; inhibitor of growth protein 1; p24ING1c; p33; p33ING1; p33ING1b; p47; p47ING1a; tumor suppressor ING1

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Citation for Human/Mouse ING1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Chromatin condensation and recruitment of PHD finger proteins to histone H3K4me3 are mutually exclusive
    Authors: Jovylyn Gatchalian, Carmen Mora Gallardo, Stephen A. Shinsky, Ruben Rosas Ospina, Andrea Mansilla Liendo, Krzysztof Krajewski et al.
    Nucleic Acids Research

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