Human/Mouse JunD Antibody

Catalog #: MAB5526
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5526
MAB5526-SP

Detection of Human/Mouse JunD by Western Blot.

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Citations (2)

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Human/Mouse JunD Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse JunD in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 512709

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human JunD
Lys47-Gly156
Accession # CAA40010

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse JunD by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse JunD Monoclonal Antibody (Catalog # MAB5526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for JunD at approximately 40 kDa and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunD

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or Fos family members to form the AP1 transcription factor. Unlike c‑Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD is a 347 amino acid (aa), 39 kDa protein (JunD-FL) that has a 303 aa, 34 kDa isoform ( delta JunD) which begins at aa 44. Over the sequence used as an immunogen, human JunD shares 72% aa identity with mouse and rat JunD.

Long Name

JunD Proto-oncogene

Entrez Gene IDs

3727 (Human); 16478 (Mouse); 24518 (Rat)

Alternate Names

activator protein 1; AP-1; jun D proto-oncogene; JunD; JunD-FL isoform; transcription factor jun-D

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human/Mouse JunD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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An integrated multi-omics approach to identify regulatory mechanisms in cancer metastatic processes
Authors: S Ghaffari, C Hanson, RE Schmidt, KJ Bouchonvil, SM Offer, S Sinha
Genome Biology, 2021-01-07;22(1):19.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.
Authors: Kashiwada M, Cassel SL, Colgan JD
EMBO J., 2011-04-15;30(10):2071-82.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot