Human/Mouse MYB Antibody Summary
Lys503-Met640
Accession # P10242
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse MYB by Western Blot. Western blot shows lysates of CEM human T-lymphoblastoid cell line, SW620 human colorectal adenocarcinoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse MYB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6209) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MYB at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MYB
MYB (myeloblastosis protooncogene) is an 80 kDa transcriptional activator. It is found in hematopoietic and intestinal crypt epithelial cells. MYB interacts with p300 and activates multiple genes, including Bcl2, GATA3, and MYC. Although MYB is thought of as a pro-proliferation and antidifferentiation factor, it would now appear that MYB contributes to both T- and B-cell maturation and to cell survival in general. Human MYB is 640 amino acids (aa) in length. It contains three DNA binding, HTH‑myb type domains (aa 35‑193), one transcriptional activation segment (aa 275‑327), and a Leu‑zipper motif (aa 376‑397). There are multiple potential splice forms. One shows an alternate start site at Met189 coupled with a 99 aa substitution for aa 567‑640. Another shows a 121 aa insertion after Ser401. Four show C‑terminal substitutions: a 34 aa substitution for aa 314‑640, a Met substitution for aa 402‑640, a seven aa substitution for aa 317‑640, and a 10 aa substitution for aa 603‑640. Over aa 503‑640, human MYB shares 84% aa identity with mouse MYB.
Product Datasheets
Citation for Human/Mouse MYB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Causes and Consequences of miR-150-5p Dysregulation in Myasthenia Gravis
Authors: Mélanie A. Cron, Solène Maillard, Frédérique Truffault, Ambra Vittoria Gualeni, Annunziata Gloghini, Elie Fadel et al.
Frontiers in Immunology
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