Human/Mouse PKC beta 2 Antibody

Catalog #: MAB43781 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43781-100
MAB43781-SP

Detection of Human and Mouse PKC beta 2 by Western Blot.

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All PKC beta 2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PKC beta 2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human PKC beta 2 in direct ELISAs. Detects human and mouse PKC beta 2 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 469827

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKC beta 2
Lys607-Ser673
Accession # P05771

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse PKC beta 2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CEM human T-lymphoblastoid cell line, and TK-1 mouse T cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKC beta 2 Monoclonal Antibody (Catalog # MAB43781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PKC beta 2 in Human Spleen. PKC beta 2 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human/Mouse PKC beta 2 Monoclonal Antibody (Catalog # MAB43781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse/Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC beta 2

Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their co‑factor requirements: conventional PKC isoforms ( alpha, beta Ι, beta ΙΙ, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon, eta, and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota / lambda, and zeta ), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC beta 2 regulation of c-myc expression has been shown to suppress insulin gene transcription in pancreatic beta -cells implicating PKC beta 2 for some of the beta -cell glucose toxicity found in diabetes.

Long Name

Protein Kinase C beta 2

Entrez Gene IDs

5579 (Human); 18751 (Mouse); 25023 (Rat)

Alternate Names

PKC beta 2; PKCB;PKCbeta;PKC-beta;PKCI(2);PRKCB;PRKCB1;PRKCB2;Protein kinase C beta type