Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

HRP

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Mouse IgG HRP-conjugated Antibody

Detection of Mouse IgG Primary Antibody by Western Blot

Detection of Mouse IgG Primary Antibody by Western Blot

Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IgG HRP-conjugated Antibody

Application
Recommended Usage

Western Blot

Use at a 1:1000 dilution.

Reviewed Applications

Read 5 reviews rated 4.8 using HAF018 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 μm filtered solution in PBS with stabilizers.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Mouse IgG HRP-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse IgG HRP-conjugated Antibody

For research use only

Citations for Mouse IgG HRP-conjugated Antibody

Customer Reviews for Mouse IgG HRP-conjugated Antibody (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse IgG HRP-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 5 reviews Showing All
Filter By:
Clear Filters X
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/01/2026
    PDX1 identified by Mouse HRP secondary in MCF7 cells whole cell lysate
    Mouse IgG HRP-conjugated Antibody HAF018
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/10/2025
    SOX17 ab identified by Mouse IgG HRP conjugate ab
    Mouse IgG HRP-conjugated Antibody HAF018
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tissue Lysates and Colon tissue
    Species: Mouse
    Verified Customer | Posted 11/27/2021
    Mouse IgG HRP-conjugated Antibody HAF018
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells and HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 08/05/2020
    Mouse IgG HRP-conjugated Antibody HAF018
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Muscle tissue
    Species: Pig
    Verified Customer | Posted 08/14/2019
    Nitrocellulose membrane visualised after incubation of primary (primary mouse monoclonalIgG, anti-hcKMM) and secondary (anti-mouse IgG, HRP conjugated) antibody. Day 1 (left) till day 50 of decomposed pig tissue.
    Mouse IgG HRP-conjugated Antibody HAF018

There are no reviews that match your criteria.

Showing  1 - 5 of 5 reviews Showing All

FAQs for Mouse IgG HRP-conjugated Antibody

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Secondary Antibodies