Mouse IgG NorthernLights™ NL493-conjugated Antibody
R&D Systems | Catalog # NL009
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Immunocytochemistry
Label
NL493 (Excitation = 493 nm, Emission = 514 nm)
Antibody Source
Polyclonal Donkey IgG
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Product Specifications
Immunogen
Mouse IgG
Specificity
Mouse IgG (H+L)
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Mouse IgG NorthernLights™ NL493-conjugated Antibody
Detection Mouse IgG Primary Antibody by Immunocytochemistry.
Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).Applications for Mouse IgG NorthernLights™ NL493-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Use at a 1:200 dilution
Immunocytochemistry
Use at a 1:200 dilution
Immunohistochemistry
Use at a 1:200 dilution
Reviewed Applications
Read 3 reviews rated 5 using NL009 in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Mouse IgG NorthernLights™ NL493-conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IgG NorthernLights™ NL493-conjugated Antibody
For research use only
Citations for Mouse IgG NorthernLights™ NL493-conjugated Antibody
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Customer Reviews for Mouse IgG NorthernLights™ NL493-conjugated Antibody (3)
5 out of 5
3 Customer Ratings
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Customer Images
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Application: Western BlotSample Tested: 4T1 mouse breast cancer cell lineSpecies: MouseVerified Customer | Posted 05/16/2019
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Application: Western BlotSample Tested: Cartilage tissueSpecies: MouseVerified Customer | Posted 05/16/2019Works great as a secondary antibody on western blots and Immunofluorescences. We used at 1:100 for western on many different mouse antibodies.
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Cartilage tissueSpecies: MouseVerified Customer | Posted 03/26/2019We used this on a cell culture in which cells were adhered to slides and stained for individual proteins. This secondary was used 1:100 for 1 h at room temperature, and worked perfectly. Was able to read on our fluorescence microscope.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse IgG NorthernLights™ NL493-conjugated Antibody
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Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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