Home / AlphaB Crystallin/CRYAB / Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

Catalog #: MAB4849
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4849
MAB4849-SP
Detection of Human, Mouse, and Rat AlphaB Crystallin/CRYAB by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human AlphaB Crystallin/CRYAB in direct ELISAs and human, mouse, and rat AlphaB Crystallin/CRYAB in Western blots. In direct ELISAs, no cross-reactivity with recombinant human AlphaA Crystallin/CRYAA is observed.
Source
Monoclonal Mouse IgG2B Clone # 731502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Crystallin/CRYAB
Met1-Lys175
Accession # P02511
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat AlphaB Crystallin/CRYAB antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat AlphaB Crystallin/CRYAB by Western Blot. Western blot shows lysates of rat heart tissue, mouse heart tissue, and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human AlphaB Crystallin/CRYAB and recombinant human AlphaA Crystallin/CRYAA (5 ng/lane) were included. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human AlphaB Crystallin/CRYAB antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human AlphaB Crystallin/CRYAB by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 28 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AlphaB Crystallin/CRYAB

CRY-alpha B (crystalline alpha -B chain/CRYAB; also HspB5) is a 22-23 kDa member of the HSP20 family of proteins.  It has widespread expression, and is found in lens epithelium where it noncovalently oligomerizes with CRYAA to generate a transparent 350-1000 kDa alpha -crystalline protein complex.  Human CRYAB is 175 amino acids (aa) in length. It contains an alpha -crystalline Hsp domain over aa 66-149.  Multiple post-translational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved.  It may also be phosphorylated at Ser45 and 59, be potentially O-GlcNAc modified at Thr158, 162 or 170, and acetylated at Lys92.  An alternate start site at Met68 may be accompanied by a 47 aa substitution for aa 109-175.  Full-length human CRYAB shares 54% aa sequence identity with CRYAA, and 98% aa identity with mouse CRYAB.  

Entrez Gene IDs
1410 (Human); 12955 (Mouse); 25420 (Rat)
Alternate Names
alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component

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Citations for Human/Mouse/Rat AlphaB Crystallin/CRYAB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space
    Authors: E Adriaensse, B Asselbergh, P Rivera-Mej, S Bervoets, L Vendredy, V De Winter, K Spaas, R de Rycke, G van Isterd, F Impens, T Langer, V Timmerman
    Nature Cell Biology, 2023-01-23;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A weakened interface in the P182L variant of HSP27 associated with severe Charcot‐Marie‐Tooth neuropathy causes aberrant binding to interacting proteins
    Authors: T Reid Reid Alderson, Elias Adriaenssens, Bob Asselbergh, Iva Pritišanac, Jonas Van Lent, Heidi Y Gastall et al.
    The EMBO Journal
  3. The bradykinin system in stress and anxiety in humans and mice
    Authors: A Rouhiainen, N Kulesskaya, M Mennesson, Z Misiewicz, T Sipilä, E Sokolowska, K Trontti, L Urpa, W McEntegart, S Saarnio, P Hyytiä, I Hovatta
    Sci Rep, 2019-12-19;9(1):19437.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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