Human/Mouse/Rat beta -Catenin Antibody Summary
Ala2-Leu781
Accession # P35222
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of beta-Catenin in Human Colon Cancer via Multiplex Immunofluorescence staining on COMET™ beta-Catenin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human beta-Catenin Monoclonal Antibody (Catalog # MAB1329) at 15 µg/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cell membrane. Protocol available in COMET™ Panel Builder.
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Detection of Human/Mouse/Rat beta -Catenin by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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beta ‑Catenin in Human Pancreas. beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Catenin
beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved in the canonical Wnt signal transduction pathway.
Product Datasheets
Citations for Human/Mouse/Rat beta -Catenin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Evaluation of a Tumor-Targeting, Near-Infrared Fluorescent Peptide for Early Detection and Endoscopic Resection of Polyps in a Rat Model of Colorectal Cancer
Authors: Jade E. Jones, Susheel Bhanu Busi, Jonathan B. Mitchem, James M. Amos-Landgraf, Michael R. Lewis
Mol Imaging
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Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
Oncogene, 2019-10-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis.
Authors: Peng J, Lai Z, Fang Z, Xing S, Hui K, Hao C, Jin Q, Qi Z, Shen W, Dong Q, Bing Z, Fu D
PLoS ONE, 2014-11-13;9(11):e112744.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Insulin and LiCl synergistically rescue myogenic differentiation of FoxO1 over-expressed myoblasts.
Authors: Wu, Yi Ju, Fang, Yen Hsin, Chi, Hsiang C, Chang, Li Chiun, Chung, Shih Yin, Huang, Wei Chie, Wang, Xiao Wen, Lee, Kuan Wei, Chen, Shen Lia
PLoS ONE, 2014-02-13;9(2):e88450.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Chronic oxidative stress causes amplification and overexpression of ptprz1 protein tyrosine phosphatase to activate beta-catenin pathway.
Authors: Liu YT, Shang D, Akatsuka S, Ohara H, Dutta KK, Mizushima K, Naito Y, Yoshikawa T, Izumiya M, Abe K, Nakagama H, Noguchi N, Toyokuni S
Am. J. Pathol., 2007-11-30;171(6):1978-88.
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P
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