Human/Mouse/Rat Catalase Antibody Summary
Met1-Leu527
Accession # P04040
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Catalase by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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Detection of Human Catalase by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Catalase in HL‑60 Human Cell Line. Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Western Blot Shows Human Catalase Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Catalase
Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H2O2, into water and O2. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.
Product Datasheets
Citations for Human/Mouse/Rat Catalase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Two Argan Oil Phytosterols, Schottenol and Spinasterol, Attenuate Oxidative Stress and Restore LPS-Dysregulated Peroxisomal Functions in Acox1−/− and Wild-Type BV-2 Microglial Cells
Authors: Soukaina Essadek, Catherine Gondcaille, Stéphane Savary, Mohammad Samadi, Joseph Vamecq, Gérard Lizard et al.
Antioxidants (Basel)
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Dynamics of antioxidant heme oxygenase-1 and pro-oxidant p66Shc in promoting advanced prostate cancer progression
Authors: Dannah R. Miller, Matthew A. Ingersoll, Yu-Wei Chou, Elizabeth A. Kosmacek, Rebecca E. Oberley-Deegan, Ming-Fong Lin
Free Radical Biology and Medicine
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Effects of a Short-Term Lipopolysaccharides Challenge on Mouse Brain and Liver Peroxisomal Antioxidant and beta -oxidative Functions: Protective Action of Argan Oil
Authors: Soukaina Essadek, Habiba Bouchab, Riad El Kebbaj, Catherine Gondcaille, Soufiane El Kamouni, Stéphane Savary et al.
Pharmaceuticals (Basel)
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Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif
Authors: MA Farelo, D Korrou-Kar, KF Brooks, TA Russell, K Maringer, PU Mayerhofer
Viruses, 2022-01-27;14(2):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates De Novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation
Authors: Kohei Hattori, Kenji Tago, Shiori Memezawa, Arisa Ochiai, Sui Sawaguchi, Yukino Kato et al.
Medicines (Basel)
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Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases
Authors: A Yammine, A Zarrouk, T Nury, A Vejux, N Latruffe, D Vervandier, M Samadi, JJ Mackrill, H Greige-Ger, L Auezova, G Lizard
Cells, 2020-10-23;9(11):.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Olive oil with high polyphenolic content induces both beneficial and harmful alterations on rat redox status depending on the tissue
Authors: P Kouka, F Tekos, Z Papoutsaki, P Stathopoul, M Halabalaki, M Tsantarlio, I Zervos, C Nepka, J Liesivuori, VN Rakitskii, A Tsatsakis, AS Veskoukis, D Kouretas
Toxicol Rep, 2020-02-13;7(0):421-432.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Therapeutic Effects of Nrf2 Activation by Bardoxolone Methyl in Chronic Heart Failure
Authors: Changhai Tian, Lie Gao, Andi Zhang, Bryan T. Hackfort, Irving H. Zucker
Journal of Pharmacology and Experimental Therapeutics
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p66Shc protein through a redox mechanism enhances the progression of prostate cancer cells towards castration-resistance
Authors: Dannah R. Miller, Matthew A. Ingersoll, Arpita Chatterjee, Brian Baker, Shashank Shrishrimal, Elizabeth A. Kosmacek et al.
Free Radical Biology and Medicine
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Mitochondrial function in liver cells is resistant to perturbations in NAD+ salvage capacity
Authors: M Dall, SAJ Trammell, M Asping, AS Hassing, M Agerholm, SG Vienberg, MP Gillum, S Larsen, JT Treebak
J. Biol. Chem., 2019-07-18;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells
Authors: NIKOLAOS GOUTZOURELAS, DIMITRIOS STAGOS, ANASTASIA HOUSMEKERIDOU, CHRISTINA KARAPOULIOU, EFTHALIA KERASIOTI, NEKTARIOS ALIGIANNIS et al.
International Journal of Molecular Medicine
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AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD
Authors: Josef Brandauer, Marianne A. Andersen, Holti Kellezi, Steve Risis, Christian Frøsig, Sara G. Vienberg et al.
Frontiers in Physiology
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Glutathione transferases P1/P2 regulate the timing of signaling pathway activations and cell cycle progression during mouse liver regeneration
Authors: J Pajaud, C Ribault, I Ben Mosbah, C Rauch, C Henderson, P Bellaud et al.
Cell Death & Disease
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A critical role of autophagy in antileukemia/lymphoma effects of APO866, an inhibitor of NAD biosynthesis.
Authors: Ginet, Vanessa, Puyal, Julien, Rummel, Coralie, Aubry, Dominiqu, Breton, Caroline, Cloux, Anne-Jul, Majjigapu, Somi R, Sordat, Bernard, Vogel, Pierre, Bruzzone, Santina, Nencioni, Alessio, Duchosal, Michel A, Nahimana, Aimable
Autophagy, 2014-01-17;10(4):603-17.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4
Authors: Kwon IK, Wang R, Thangaraju M
Oncogene, 2010-03-29;29(0):3423.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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we overexpressed catalase contained plasmid in HEK293 cells and used 1:1000 dilution to detect this antibody.
