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Human/Mouse/Rat EEA1 Antibody

Catalog #: AF8047
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8047
AF8047-SP
Detection of Human, Mouse, and Rat EEA1 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat EEA1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat EEA1 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EEA1
Asn1249-Gln1356
Accession # Q15075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat EEA1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat EEA1 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and Sheep Anti-Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat EEA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8047) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EEA1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EEA1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

EEA1 in HeLa Human Cell Line. EEA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat EEA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8047) at 1.7 and 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to early endosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EEA1

EEA1 (Early Endosome Autoantigen 1; also Endosome-associated protein p162 and Zn-finger FYVE domain-containing protein 2) is a 170-180 kDa protein that serves as an identifying marker for early endosomes. It is ubiquitously expressed, and found in both the cytosol and on cellular membranes. Its activity has been described as that of a tethering factor which links endosomes to endocytic vesicles, allowing for their fusion via a SNARE complex. Normally, EEA1 exists as a homodimer in the cytoplasm and appears to make transient contacts with endosome membrane phosphatidylinositol. When endosome fusion is not required, EEA1 serves as a substrate for p97, promoting EEA1 dissociation and endosome independence. When endosome fusion is required, EEA1 interacts with NSF, resulting in its removal from a large endosome-associated complex and subsequent endosomal vesicle fusion. Human EEA1 is synthesized as a 1411 amino acid (aa) protein that contains one C2H2-type Zn finger region (aa 41-64) and one FYVE Zn finger domain (aa 1352-1410). There is one isoform variant that contains a nine aa substitution for aa 925-1411. Over aa 1249-1356, human EEA1 shares 99% aa sequence identity with mouse EEA1.

Long Name
Early Endosome Antigen 1
Entrez Gene IDs
8411 (Human); 216238 (Mouse); 314764 (Rat)
Alternate Names
early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; ZFYVE2MST105; Zinc finger FYVE domain-containing protein 2

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Citations for Human/Mouse/Rat EEA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Proton-gated anion transport governs macropinosome shrinkage
    Authors: Mariia Zeziulia, Sandy Blin, Franziska W. Schmitt, Martin Lehmann, Thomas J. Jentsch
    Nature Cell Biology
  2. SIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis model
    Authors: M Morell, N Varela, C Castillejo, C Coppard, MJ Luque, YY Wu, N Martín-Mor, F Pérez-Cóza, G Gómez-Hern, R Kumar, F O'Valle, ME Alarcón-Ri, C Marañón
    EBioMedicine, 2022-01-19;76(0):103808.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Allosteric inhibition of SHP2 uncovers aberrant TLR7 trafficking in aggravating psoriasis
    Authors: Y Zhu, Z Wu, W Yan, F Shao, B Ke, X Jiang, J Gao, W Guo, Y Lai, H Ma, D Chen, Q Xu, Y Sun
    Embo Molecular Medicine, 2021-12-22;0(0):e14455.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells
    Authors: JAF Silva, X Qi, MB Grant, ME Boulton
    Scientific Reports, 2021-08-30;11(1):17400.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. EGFL7 enhances surface expression of integrin ?5?1 to promote angiogenesis in malignant brain tumors
    Authors: N Dudvarski, F Bicker, S Keller, DT Jones, PN Harter, A Kienzle, C Gillmann, P Arnold, A Golebiewsk, O Keunen, A Giese, A von Deimli, T Bäuerle, SP Niclou, M Mittelbron, W Ye, SM Pfister, MH Schmidt
    EMBO Mol Med, 2018-09-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABAA receptors
    Authors: JiaJie Teoh, Narayan Subramanian, Maria Elena Pero, Francesca Bartolini, Ariadna Amador, Ayla Kanber et al.
    Neurobiology of Disease
  7. Extracellular clusterin limits the uptake of alpha-synuclein fibrils by murine and human astrocytes
    Authors: Filippini A, Mutti V, Faustini G et al.
    Glia

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