Human/Mouse/Rat Galectin-3 Antibody

Detection of Mouse Galectin‑3 by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.25 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Rat Galectin‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U-118-MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Galectin‑3 by Simple Western^TM. Simple Western lane view shows lysates of HT-2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 43 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Galectin-3 by Western Blot A beta oligomerization and Gal-3 expression are age-dependently increased in APP/PS1 mice. a Wild-type (3 months old) and APP/PS1 mice of different ages (3, 5, 8, and 11 months) were sacrificed and dissected hippocampal tissues were subjected to Western blot analysis for endogenous A beta oligomers. b Quantified results for HMW and LMW A beta oligomerization [n = 4 per group; F(4,15) = 39.3, P < 0.001 for HMW and F(4,15) = 74.07, P < 0.001 for LMW]. Statistical significances for various comparisons are shown in the figure. c Western blot analysis showing the expression levels of Gal-3 and PIAS1 in hippocampal samples from the same batch of WT mice and APP/PS1 mice of different ages (n = 4 per group). d Quantified results for Gal-3 expression in WT and APP/PS1 mice [F(4,15) = 14.59, P < 0.001]. e Quantified results for PIAS1 expression in WT and APP/PS1 mice [F(4,15) = 5.86, P < 0.01] The letter “m” indicates month. Statistical significances of various comparisons are shown in the figure. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, #P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Galectin-3 by Western Blot A beta oligomerization and Gal-3 expression are increased in AD patients. The frontal lobe lysates of normal subjects and AD patients were subjected to Western blot analysis for A beta oligomerization and the expression of Gal-3 and Gal-1 (n = 4 per group). b–d Quantified results for (b) HMW [t(1,6) = 9.72, P < 0.001] and LMW [t(1,6) = 7.21, P < 0.001] A beta oligomerization, c Gal-3 expression [t(1,6) = 4.92, P < 0.01] and d Gal-1 expression [t(1,6) = 0.43, P > 0.05]. e Immunohistochemical analysis showing the distributions of Gal-3 and ProteoStat staining in normal subjects and AD patients (n = 3 per group). Scale bar, 25 μm. DAPI was used to stain nuclei. Data are expressed as mean ± SEM. **P < 0.01, #P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Galectin-3 by Western Blot Endogenous Gal-3 expression is decreased but memory performance is improved in APP/PS1;Gal-3+/− mice. a Endogenous expression levels of Gal-3 and NEP were examined by Western blot analysis in 3-month-old WT;WT, APP/PS1;WT, WT;Gal-3−/− and APP/PS1;Gal-3+/− mice. b Quantified results for Gal-3 and NEP expression [n = 4 per group; for Gal-3, F(3,12) = 53.47, P < 0.001; q = 10.43, P < 0.001 for the WT;WT group versus the APP/PS1;WT group; q = 6.46, P < 0.001 for the APP/PS1;WT group versus the APP/PS1;Gal-3+/− group and q = 3.98, P < 0.05 for the WT;WT group versus the APP/PS1;Gal-3+/− group; for NEP, F(3,12) = 27.76, P < 0.001; q = 12.04, P < 0.001 for the WT;WT group versus the APP/PS1;WT group; q = 9.72, P < 0.001 for the WT;WT group versus the WT;Gal-3−/− group and q = 6.29, P < 0.01 for the WT;WT group versus the APP/PS1;Gal-3+/− group]. c Acquisition performance obtained by assessing the water maze learning of 8-month-old mice of the four genotypes [n = 7 per group; F(3,24) = 28.72, P < 0.001; q = 9.33, P < 0.001 for the APP/PS1;WT group versus the WT;WT group and q = 3.59, P < 0.05 for the APP/PS1;Gal-3+/− group versus the APP/PS1;WT group]. d Retention performance of the same mice described in (c) [n = 7 per group; F(3,24) = 4.76, P < 0.01; q = 3.94, P < 0.05 for the APP/PS1;WT group versus the WT;WT group; q = 3.25, P < 0.05 for the APP/PS1;Gal-3+/− group versus the APP/PS1;WT group for the target quadrant]. Representative swim patterns from each group are also shown (upper panel). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, #P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Galectin-3 by Western Blot Galectin-3 associates with A beta and interacts with A beta. a Immunohistochemical analysis showing the distributions of endogenous Gal-3 and A beta in hippocampal samples of WT (left panel) and APP/PS1 (right panel) mice at 11 months of age. Scale bar, 200 μm. b Co-IP experiment showing preferential association of Gal-3 with endogenous A beta monomers in 8-month-old APP/PS1 mice. Experiments were performed in duplicate. c Different amounts of recombinant human Gal-3 protein (0.25, 0.5, 1, and 2 μg) were added to a solution containing A beta 42 peptide (15 μg), the mixture was subjected to a thioflavin-T assay, and fluorescence was measured hourly at different time points. The fluorescence plateaued at the 7-h time point. Experiments were performed in triplicate. The abbreviation “rh” indicates “recombinant human”. Data are expressed as mean ± SEM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Galectin-3 by Immunohistochemistry Tumor derived galectin-3 is a ligand for TREM2. (C) Immunohistochemical staining of galectin-3 in adjacent normal lung & intratumor areas of lung tissues (n = 5). Scale bars, 20 μm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39135069), licensed under a CC-BY license. Not internally tested by R&D Systems.