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Human/Mouse/Rat Galectin-3 Antibody

Catalog #: AF1197
41 Citations 1 Review Datasheet / COA / SDS
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AF1197
AF1197-SP
Detection of Mouse Galectin‑3 by Western Blot.
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Product Details
Citations (41)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Galectin-3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Galectin-3 in Western blots. Detects mouse Galectin-3 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Galectin-3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Galectin-4 and rmGalectin-9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Galectin‑3
Ala2-Ile264
Accession # NP_034835
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05-0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Galectin-3 antibody by Western Blot. View Larger

Detection of Mouse Galectin‑3 by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human and Rat Galectin-3 antibody by Western Blot. View Larger

Detection of Human and Rat Galectin‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U-118-MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Galectin‑3 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Galectin‑3 in Mouse Embryo. Galectin-3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197 ) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Galectin-3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Galectin‑3 by Simple WesternTM. Simple Western lane view shows lysates of HT-2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3 at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has proinflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity (1-5).

References
  1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2001) Biochimie 83:667.
  4. R&D Systems Cytokine Bulletin; Summer 2002.
  5. Gorski, J.P. et al. (2002) J. Biol. Chem. 277:18840.
Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

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Citations for Human/Mouse/Rat Galectin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Hyperglycemia Induces Trained Immunity in Macrophages and Their Precursors and Promotes Atherosclerosis
    Authors: Laurienne Edgar, Naveed Akbar, Adam T. Braithwaite, Thomas Krausgruber, HĂ©ctor Gallart-Ayala, Jade Bailey et al.
    Circulation
  2. Apolipoprotein E4 impairs the response of neurodegenerative retinal microglia and prevents neuronal loss in glaucoma
    Authors: Milica A. Margeta, Zhuoran Yin, Charlotte Madore, Kristen M. Pitts, Sophia M. Letcher, Jing Tang et al.
    Immunity
  3. A key role for the novel coronary artery disease gene JCAD in atherosclerosis via shear stress mechanotransduction
    Authors: Gillian Douglas, Vedanta Mehta, Ayman Al Haj Zen, Ioannis Akoumianakis, Anuj Goel, Victoria S Rashbrook et al.
    Cardiovascular Research
  4. Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington’s disease
    Authors: Jian Jing Siew, Hui-Mei Chen, Huan-Yuan Chen, Hung-Lin Chen, Chiung-Mei Chen, Bing-Wen Soong et al.
    Nature Communications
  5. SerpinB3 as a Pro-Inflammatory Mediator in the Progression of Experimental Non-Alcoholic Fatty Liver Disease
    Authors: Erica Novo, Andrea Cappon, Gianmarco Villano, Santina Quarta, Stefania Cannito, Claudia Bocca et al.
    Frontiers in Immunology
  6. Nurr1 repression mediates cardinal features of Parkinson’s disease in alpha -synuclein transgenic mice
    Authors: Maria Argyrofthalmidou, Athanasios D Spathis, Matina Maniati, Amalia Poula, Maira A Katsianou, Evangelos Sotiriou et al.
    Human Molecular Genetics
  7. The role of P2Y12 in the kinetics of microglial self-renewal and maturation in the adult visual cortex in vivo
    Authors: Monique S Mendes, Linh Le, Jason Atlas, Zachary Brehm, Antonio Ladron-de-Guevara, Evelyn Matei et al.
    eLife
  8. Galectin-3 Negatively Regulates Hippocampus-Dependent Memory Formation through Inhibition of Integrin Signaling and Galectin-3 Phosphorylation
    Authors: Yan-Chu Chen, Yun-Li Ma, Cheng-Hsiung Lin, Sin-Jhong Cheng, Wei-Lun Hsu, Eminy H.-Y. Lee
    Frontiers in Molecular Neuroscience
  9. Temporal changes in glucose metabolism reflect polarization in resident and monocyte-derived macrophages after myocardial infarction
    Authors: Alan J. Mouton, Nikaela M. Aitken, Sydney P. Moak, Jussara M. do Carmo, Alexandre A. da Silva, Ana C. M. Omoto et al.
    Frontiers in Cardiovascular Medicine
  10. Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue
    Authors: Uff CEG, Patel K, Yeung C, Yip PK
    Biomolecules
  11. Apelin/APJ relieve diabetic cardiomyopathy by reducing microvascular dysfunction
    Authors: Bin Li, Jiming Yin, Jing Chang, Jia Zhang, Yangjia Wang, Haixia Huang et al.
    Journal of Endocrinology
  12. Engineering an inhibitor-resistant human CSF1R variant for microglia replacement
    Authors: Jean Paul Chadarevian, Sonia I. Lombroso, Graham C. Peet, Jonathan Hasselmann, Christina Tu, Dave E. Marzan et al.
    Journal of Experimental Medicine
  13. Galectin-3 activates spinal microglia to induce inflammatory nociception in wild type but not in mice modelling Alzheimer's disease
    Authors: Sideris-Lampretsas, G;Oggero, S;Zeboudj, L;Silva, R;Bajpai, A;Dharmalingam, G;Collier, DA;Malcangio, M;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates, Whole Tissue
    Applications: Western Blot, IHC
  14. Mutation of the galectin-3 glycan-binding domain (Lgals3-R200S) enhances cortical bone expansion in male mice and trabecular bone mass in female mice
    Authors: KA Maupin, CR Diegel, PD Stevens, D Dick, VAI Vivari, BO Williams
    FEBS Open Bio, 2022-09-14;0(0):.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  15. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia
    Authors: H Shi, Z Yin, Y Koronyo, DT Fuchs, J Sheyn, MR Davis, JW Wilson, MA Margeta, KM Pitts, S Herron, S Ikezu, T Ikezu, SL Graham, VK Gupta, KL Black, M Mirzaei, O Butovsky, M Koronyo-Ha
    Acta neuropathologica communications, 2022-09-08;10(1):136.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Crosstalk between dihydroceramides produced by Porphyromonas gingivalis and host lysosomal cathepsin B in the promotion of osteoclastogenesis
    Authors: C Duarte, C Yamada, C Garcia, J Akkaoui, A Ho, F Nichols, A Movila
    Journal of Cellular and Molecular Medicine, 2022-04-16;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IFC, Western Blot
  17. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
    Authors: T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu
    Nature Communications, 2022-03-03;13(1):1134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Gal3 Plays a Deleterious Role in a Mouse Model of Endotoxemia
    Authors: JC Fernández-, AM Espinosa-O, I García-Dom, I Rosado-Sán, YM Pacheco, R Moyano, JG Monterde, JL Venero, RM de Pablos
    International Journal of Molecular Sciences, 2022-01-21;23(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease
    Authors: C Emre, KV Do, B Jun, E Hjorth, SG Alcalde, MI Kautzmann, WC Gordon, P Nilsson, NG Bazan, M Schultzber
    Acta neuropathologica communications, 2021-06-29;9(1):116.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3
    Authors: NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann
    Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
    Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
    Acta Neuropathol Commun, 2020-10-07;8(1):163.
    Species: Mouse
    Sample Types: Protein, Whole Tissue
    Applications: IHC-P, Western Blot
  22. Serum amyloid A-containing HDL binds adipocyte-derived versican and macrophage-derived biglycan, reducing its anti-inflammatory properties
    Authors: CY Han, I Kang, M Omer, S Wang, T Wietecha, TN Wight, A Chait
    JCI Insight, 2020-09-24;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Plasma APE1/Ref-1 Correlates with Atherosclerotic Inflammation in ApoE-/- Mice
    Authors: YR Lee, HK Joo, EO Lee, MS Park, HS Cho, S Kim, H Jin, JO Jeong, CS Kim, BH Jeon
    Biomedicines, 2020-09-21;8(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  24. Adipocyte-Derived Versican and Macrophage-Derived Biglycan Control Adipose Tissue Inflammation in Obesity
    Authors: CY Han, I Kang, IA Harten, JA Gebe, CK Chan, M Omer, KM Alonge, LJ den Hartig, D Gomes Kjer, L Goodspeed, S Subramania, S Wang, F Kim, DE Birk, TN Wight, A Chait
    Cell Rep, 2020-06-30;31(13):107818.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Voluntary running does not reduce neuroinflammation or improve non-cognitive behavior in the 5xFAD mouse model of Alzheimer's disease
    Authors: M Svensson, E Andersson, O Manouchehr, Y Yang, T Deierborg
    Sci Rep, 2020-01-28;10(1):1346.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  26. Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes
    Authors: T Iwanaga, J Nio-Kobaya, H Takahashi-
    Biomed. Res., 2019-01-01;40(2):79-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Peripheral Inflammation Enhances Microglia Response and Nigral Dopaminergic Cell Death in an in vivo MPTP Model of Parkinson's Disease
    Authors: I García-Dom, K Veselá, J García-Rev, A Carrillo-J, MA Roca-Cebal, M Santiago, RM de Pablos, JL Venero
    Front Cell Neurosci, 2018-11-06;12(0):398.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection
    Authors: A Alawieh, M Andersen, DL Adkins, S Tomlinson
    J. Neurosci., 2018-06-19;38(29):6527-6545.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy
    Authors: A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga
    Exp. Eye Res., 2018-03-27;172(0):1-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Identifying the role of complement in triggering neuroinflammation after traumatic brain injury
    Authors: A Alawieh, EF Langley, S Weber, D Adkins, S Tomlinson
    J. Neurosci., 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Kr�ppel-like factor 3 (KLF3/BKLF) is required for widespread repression of the inflammatory modulator galectin-3 (Lgals3)
    J Biol Chem, 2016-05-24;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: IHC-P, Western Blot
  32. Galectin-1 and galectin-8 have redundant roles in promoting plasma cell formation.
    Authors: Tsai CM, Guan CH, Hsieh HW, Hsu TL, Tu Z, Wu KJ, Lin CH, Lin KI
    J. Immunol., 2011-07-13;187(4):1643-52.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury.
    Authors: Buhimschi CS, Baumbusch MA, Dulay AT, Oliver EA, Lee S, Zhao G, Bhandari V, Ehrenkranz RA, Weiner CP, Madri JA, Buhimschi IA
    Am. J. Pathol., 2009-08-13;175(3):958-75.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction.
    Authors: Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, Greaves DR
    Arterioscler. Thromb. Vasc. Biol., 2007-12-20;28(3):433-40.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  35. Bone Marrow Stromal Cell Antigen 2: Is a Potential Neuroinflammation Biomarker of SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis in Pre-symptomatic Stage
    Authors: Xiaojiao Xu, Jingjing Zhang, Song Li, Murad Al-Nusaif, Qinming Zhou, Sheng Chen et al.
    Frontiers in Neuroscience
  36. Targeting galectin-3 to counteract spike-phase uncoupling of fast-spiking interneurons to gamma oscillations in Alzheimer’s disease
    Authors: Luis Enrique Arroyo-GarcĂ­a, Sara Bachiller, RocĂ­o Ruiz, Antonio Boza-Serrano, Antonio RodrĂ­guez-Moreno, Tomas Deierborg et al.
    Translational Neurodegeneration
  37. IL-12 alpha deficiency attenuates pressure overload-induced cardiac inflammation, hypertrophy, dysfunction, and heart failure progression
    Authors: Umesh Bhattarai, Xiaochen He, Rui Xu, Xiaoguang Liu, Lihong Pan, Yuxiang Sun et al.
    Frontiers in Immunology
  38. The Diagnostic and Prognostic Value of Plasma Galectin 3 in HFrEF Related to the Etiology of Heart Failure
    Authors: Qun Lu, Ruo-Chen Zhang, Shu-Ping Chen, Tao Li, Ya Wang, Yan-Bo Xue et al.
    Frontiers in Cardiovascular Medicine
  39. Regulation of mycobacterial infection by macrophage Gch1 and tetrahydrobiopterin
    Authors: Eileen McNeill, Elena Stylianou, Mark J. Crabtree, Rachel Harrington-Kandt, Anna-Lena Kolb, Marina Diotallevi et al.
    Nature Communications
  40. Modulation of the Cellular Expression of Circulating Advanced Glycation End-Product Receptors in Type 2 Diabetic Nephropathy
    Authors: Karly C. Sourris, Brooke E. Harcourt, Sally A. Penfold, Felicia Y. T. Yap, Amy L. Morley, Philip E. Morgan et al.
    Experimental Diabetes Research
  41. Galectin-3 deficiency ameliorates fibrosis and remodeling in dilated cardiomyopathy mice with enhanced Mst1 signaling
    Authors: My-Nhan Nguyen, Mark Ziemann, Helen Kiriazis, Yidan Su, Zara Thomas, Qun Lu et al.
    American Journal of Physiology-Heart and Circulatory Physiology

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Human/Mouse/Rat Galectin-3 Antibody
By Anonymous on 01/24/2024
Application: Immunocytochemistry/Immunofluorescence
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Galectin-3 Antibody AF1197