Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunocytochemistry, Co-Immunoprecipitation, Simple Plex Automated Immunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 686613
View all GAPDH Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GAPDH/G3PDH
Met1-Ala150
Accession # P04406

Specificity

Detects human, mouse and rat GAPDH/G3PDH in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse GAPDH/G3PDH and no cross-reactivity with recombinant human GAPDH-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat GAPDH Antibody

Detection of Human, Mouse, and Rat GAPDH/G3PDH antibody by Western Blot.

Detection of Human, Mouse, and Rat GAPDH/G3PDH by Western Blot.

Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAPDH/G3PDH at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
GAPDH antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

GAPDH in HeLa Human Cell Line.

GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat GAPDH Monoclonal Antibody (Catalog # MAB5718) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GAPDH/G3PDH antibody in Human Kidney by Immunohistochemistry (IHC-P).

GAPDH/G3PDH in Human Kidney.

GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse GAPDH/G3PDH antibody by Simple WesternTM.

Detection of Human and Mouse GAPDH/G3PDH by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH/G3PDH at approximately 41 kDa (as indicated) using 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human, Mouse, and Rat GAPDH by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 42 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH Monoclonal Antibody (Catalog # MAB5718). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human GAPDH by Western Blot

Detection of Human GAPDH by Western Blot

Simultaneous knockdown of multiple genes in primary human islets following electroporation. (A) Graph shows RT-qPCR results of islets electroporated with non-targeting control siRNA or siRNA targeting RB and p130 (L-003299). (B) Graph shows RT-qPCR results of islets electroporated with non-targeted control siRNA or siRNAs targeting the indicated gene products. The housekeeping gene GAPDH was used as the control. Representative experiment shown from 3 separate experiments. Error bars indicate standard deviation. (C) Western blot for RB protein in human islet lysates after electroporation of control or targeting siRNA. GAPDH was used as a loading control. RB appears as a characteristic doublet representing the hypo and hyperphosphorylated protein. Representative experiment shown from 3 different experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25305068), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GAPDH by Western Blot

Detection of GAPDH by Western Blot

Evaluation of CPP/mRNA-mediated protein expression. (A) Representative fluorescence images and a quantitative graph showing mCherry-positive CT26.CL25 cells 24 h after treatment with CPP/mCherry mRNA complexes (6.8 nM). Scale bar: 275 μm. Data are presented as the mean ± SD (n = 3). (B) Representative flow cytometry histograms and graphs of EGFP-positive CL26.CL25 cells transfected with CPP/EGFP mRNA complexes (6.8 nM). Data are presented as the mean ± SD (n = 3). (C) Western blot analysis of the expression levels of OVA and GAPDH in CT26.CL25 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35745843), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GAPDH by Western Blot

Detection of GAPDH by Western Blot

Evaluation of CPP/mRNA-mediated protein expression. (A) Representative fluorescence images and a quantitative graph showing mCherry-positive CT26.CL25 cells 24 h after treatment with CPP/mCherry mRNA complexes (6.8 nM). Scale bar: 275 μm. Data are presented as the mean ± SD (n = 3). (B) Representative flow cytometry histograms and graphs of EGFP-positive CL26.CL25 cells transfected with CPP/EGFP mRNA complexes (6.8 nM). Data are presented as the mean ± SD (n = 3). (C) Western blot analysis of the expression levels of OVA and GAPDH in CT26.CL25 cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35745843), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GAPDH by Western Blot

Detection of GAPDH by Western Blot

Molecular effect of intraluminal chloride and GsMTx4 in smooth muscle cells. a-c Upper panel represents the main cumulative effect of intraluminal chloride concentration ([Cl−]) and medium supplementation with and without GsMTx4. a Examples of representative blots are shown. b, c Protein expression levels for b myosin light chain 2 (MLC2), and c alpha-smooth muscle actin ( alpha -SMA) are quantified. d–f Lower panel shows the additional effect of PIEZO1/2 inhibition after intraluminal injection of Cl− channels inhibitors: cystic fibrosis transmembrane conductance regulator inhibitor172 (CFTRinh) to CFTR and; calcium-dependent Cl− channel inhibitor A01 (CaCCinh) to CaCCs. d Examples of representative blots are shown. e–f Relative expression levels of e MLC2, and f alpha -SMA are displayed. 143 mM Cl− and standard solution (SS) represent the control condition for [Cl−] and Cl− channels inhibitors, respectively. White and dotted rectangles represent the medium supplementation with and without GsMTx4, respectively. Each lane represents a pooled-tissue sample, and the relative expression levels were determined against GAPDH. n ≥ 4 were used per antibody/condition. Results are presented as mean ± SD. Symbols indicate the main effects and non-redundant interactions of the two-way ANOVA. p < alpha 0.0001, beta 0.001, gamma 0.01, µ0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36740669), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GAPDH by Western Blot

Detection of GAPDH by Western Blot

Molecular effect of intraluminal chloride and GsMTx4 in smooth muscle cells. a-c Upper panel represents the main cumulative effect of intraluminal chloride concentration ([Cl−]) and medium supplementation with and without GsMTx4. a Examples of representative blots are shown. b, c Protein expression levels for b myosin light chain 2 (MLC2), and c alpha-smooth muscle actin ( alpha -SMA) are quantified. d–f Lower panel shows the additional effect of PIEZO1/2 inhibition after intraluminal injection of Cl− channels inhibitors: cystic fibrosis transmembrane conductance regulator inhibitor172 (CFTRinh) to CFTR and; calcium-dependent Cl− channel inhibitor A01 (CaCCinh) to CaCCs. d Examples of representative blots are shown. e–f Relative expression levels of e MLC2, and f alpha -SMA are displayed. 143 mM Cl− and standard solution (SS) represent the control condition for [Cl−] and Cl− channels inhibitors, respectively. White and dotted rectangles represent the medium supplementation with and without GsMTx4, respectively. Each lane represents a pooled-tissue sample, and the relative expression levels were determined against GAPDH. n ≥ 4 were used per antibody/condition. Results are presented as mean ± SD. Symbols indicate the main effects and non-redundant interactions of the two-way ANOVA. p < alpha 0.0001, beta 0.001, gamma 0.01, µ0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36740669), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GAPDH by Western Blot

Detection of GAPDH by Western Blot

The impact of combination therapy on protein and gene expression in SJSA-1 osteosarcoma cells. (A) A Western blot analysis illustrating the effects of the combination treatment on key proteins involved in metastasis, cell survival, and apoptosis in SJSA-1 osteosarcoma cells. The expression levels of MMP9 and MMP13, which facilitate tumor cell invasion and metastasis through ECM degradation, were significantly reduced, indicating a potential inhibitory effect of the combination therapy on metastatic progression. Additionally, phosphorylated AKT (pAKT), a central regulator of the PI3K/AKT pathway associated with cell survival and chemoresistance, and runt-related transcription factor 2 (RUNX2), a critical modulator of osteosarcoma progression and tumor aggressiveness, were both downregulated, suggesting the suppression of key oncogenic pathways. Conversely, apoptotic markers, including cleaved caspase-8, cleaved caspase-3, and total caspase-3, exhibited increased activation following treatment, indicating enhanced apoptotic signaling. GAPDH was used as a loading control to ensure equal protein loading across samples. (B) A densitometric analysis of the Western blot bands representing the expression levels of target proteins after 48 h of treatment. The band intensities were quantified using Image Lab software (Version 6.1.0 build 7) and normalized to the GAPDH. Relative protein expression levels are expressed as a fold change compared to the untreated control group. The data represent the mean ± the standard deviation from two independent experiments. (C) qRT-PCR analyzed the gene expression of the apoptosis-related markers p16 and p53 following treatment. The results highlight the upregulation of p16 and stable expression of p53 in treated cells. (* p < 0.05 and ** p < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40332124), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat GAPDH Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

0.25 - 1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line

Western Blot

0.05 µg/mL
Sample: Human brain tissue, mouse brain tissue, and rat brain tissue

Reviewed Applications

Read 4 reviews rated 5 using MAB5718 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GAPDH

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a 36-40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length. There are two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151-155. GAPDH contains no fewer that 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over
aa 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase

Alternate Names

G3PDH

Entrez Gene IDs

2597 (Human); 14433 (Mouse); 24383 (Rat)

Gene Symbol

GAPDH

UniProt

P04406 (Human)

Additional GAPDH Products

Product Documents for Human/Mouse/Rat GAPDH Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat GAPDH Antibody

For research use only

Citations for Human/Mouse/Rat GAPDH Antibody

Customer Reviews for Human/Mouse/Rat GAPDH Antibody (4)

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4 Customer Ratings
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Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat GAPDH Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lines
    Species: Human
    Verified Customer | Posted 08/05/2025
    Human/Mouse/Rat GAPDH Antibody MAB5718
  • Human/Mouse/Rat GAPDH Antibody
    Name: Lucy Quach
    Application: Western Blot
    Sample Tested: Cancer cell lysates and Xenograft Lysates
    Species: Human and Mouse
    Verified Customer | Posted 07/03/2018
  • Human/Mouse/Rat GAPDH Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 03/01/2017
    Expression of Human p75NGF in several cell lines, with GAPDH levels showing equal loading. Dilution: 1:10,000 in PBS with 5% BSA. Secondary Ab: 1:5,000 anti-mouse IgG.
    Human/Mouse/Rat GAPDH Antibody MAB5718
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24040198
    Species: Mouse
    Verified Customer | Posted 02/25/2015

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