Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Knockout Validated, Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Simple Western, COMET

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 893205
View all Gelsolin/GSN Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Gelsolin/GSN
Met1-Ala782
Accession # P06396

Specificity

Detects human Gelsolin/GSN in ELISAs. Detects human, mouse and rat Gelsolin/GSN in Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Gelsolin/GSN Antibody

Detection of Gelsolin/GSN in Human Renal Cell Carcinoma via seqIF™ staining on COMET™

Gelsolin/GSN was detected in immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma using Mouse Anti-Human Gelsolin/GSN, pan Monoclonal Antibody (Catalog # MAB8170) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
Detection of Human, Mouse, and Rat Gelsolin/GSN antibody by Western Blot.

Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot.

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Gelsolin/GSN antibody by Simple WesternTM.

Detection of Human Gelsolin/GSN by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (U‑87 MG) (NBP2-49844) and SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Gelsolin/GSN at approximately 90 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Gelsolin/GSN antibody in Human Kidney by Immunohistochemistry (IHC-P).

Gelsolin/GSN in Human Kidney.

Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Shows Human Gelsolin/GSN Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma cell line and Gelsolin/GSN knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Gelsolin/GSN at approximately 80 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for Human/Mouse/Rat Gelsolin/GSN Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

8-25 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human kidney

Knockout Validated

Gelsolin/GSN is specifically detected in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. 

Multiplex Immunofluorescence

20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Renal Cell Carcinoma

Simple Western

5-20 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (U-87 MG) (Catalog # NBP2-49844) and SK‑Mel‑28 human malignant melanoma cell line

Western Blot

0.5 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line

Reviewed Applications

Read 1 review rated 5 using MAB8170 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gelsolin/GSN

Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family.  Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm.  Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein.  Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis.  Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition.  Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.

Alternate Names

ADF, AGEL, Brevin, GSN

Entrez Gene IDs

2934 (Human); 227753 (Mouse); 296654 (Rat)

Gene Symbol

GSN

UniProt

P06396 (Human)

Additional Gelsolin/GSN Products

Product Documents for Human/Mouse/Rat Gelsolin/GSN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Gelsolin/GSN Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human/Mouse/Rat Gelsolin/GSN Antibody

Customer Reviews for Human/Mouse/Rat Gelsolin/GSN Antibody (1)

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  • Human/Mouse/Rat Gelsolin/GSN Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreas tissue
    Species: Mouse
    Verified Customer | Posted 02/09/2022
    Human/Mouse/Rat Gelsolin/GSN Antibody MAB8170

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