Home / HIF-2 alpha/EPAS1 / Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody

Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody

Catalog #: AF2997
33 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2997
AF2997-SP
HIF‑2 alpha /EPAS1 in Human Prostate Cancer Tissue.
4 Images
Product Details
Citations (33)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat HIF-2 alpha /EPAS1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse HIF-2 alpha /EPAS1
Ser542-Thr874
Accession # AAH57870
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HIF-2a/EPAS1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HIF‑2 alpha /EPAS1 in Human Prostate Cancer Tissue. HIF-2a/EPAS1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry HIF-2a/EPAS1 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr). View Larger

HIF‑2 alpha /EPAS1 in Mouse Testis. HIF-2a/EPAS1 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Human/Mouse/Rat HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human, Mouse, and Rat HIF-2a/EPAS1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 hours. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse HIF-2a/EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2a/EPAS1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse HIF-2 alpha/EPAS1 by Western Blot View Larger

Detection of Mouse HIF-2 alpha/EPAS1 by Western Blot Kidney-derived Sca-1+ cells produce Epo. (A) Epo protein levels in supernatants obtained from Sca-1+ cells cultured in normoxia or hypoxia (n = 3 biological replicates). (B) HIF-1 alpha and HIF-2 alpha protein levels in Sca-1+ kidney-derived cells after exposure to normoxia (20% O2) or hypoxia (1% O2). Numbers below the blots indicate the fold change of the ratio of HIF-1 alpha /Tubulin and HIF-2 alpha /Tubulin in 1% O2 to the respective 20% O2 control. (C) Epo RNA levels in Sca-1+ cells that were incubated either at 20% O2 of 1% O2 as indicated (n = 4 biological replicates). (D) Mesenchymal stem cell markers in renal Sca-1+ cells on day 21 of culture; reads per kilobase million (RPKM). (E) Volcano plot of 4592 significantly up- and 5376 significantly downregulated mRNAs in kidney-derived Sca-1+ cells on day 21 in culture compared to day 0. Mean values ± SEM are shown. * p < 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35203399), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.

Long Name
Hypoxia-inducible Transcription Factor 2 alpha
Entrez Gene IDs
2034 (Human); 13819 (Mouse)
Alternate Names
Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Dynamic regulation of hypoxia-inducible factor-1&alpha activity is essential for normal B cell development
    Authors: N Burrows, RJM Bashford-R, VJ Bhute, A Peñalver, JR Ferdinand, BJ Stewart, JEG Smith, M Deobagkar-, G Giudice, TM Connor, A Inaba, L Bergamasch, S Smith, MGB Tran, E Petsalaki, PA Lyons, M Espeli, BJP Huntly, KGC Smith, RJ Cornall, MR Clatworthy, PH Maxwell
    Nat. Immunol., 2020-08-31;0(0):.
  2. HIF-1alpha-PDk1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity.
    Authors: Semba H, Takeda N, Isagawa T et al.
    Nat Commun.
  3. Targeted Disruption of the MORG1 Gene in Mice Causes Embryonic Resorption in Early Phase of Development
    Authors: Wulf, S;Mizko, L;Herrmann, KH;Sánchez-Carbonell, M;Urbach, A;Lemke, C;Berndt, A;Loeffler, I;Wolf, G;
    Biomolecules
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Hypoxia promotes an inflammatory phenotype of fibroblasts in pancreatic cancer
    Authors: AM Mello, T Ngodup, Y Lee, KL Donahue, J Li, A Rao, ES Carpenter, HC Crawford, M Pasca di M, KE Lee
    Oncogenesis, 2022-09-15;11(1):56.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. The HIFalpha-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells
    Authors: A Jatho, A Zieseniss, K Brechtel-C, J Guo, KO Böker, G Salinas, RH Wenger, DM Katschinsk
    Cells, 2022-02-21;11(4):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The renal cancer risk allele at 14q24.2 activates a novel hypoxia-inducible transcription factor-binding enhancer of DPF3 expression
    Authors: J Protze, S Naas, R Krüger, C Stöhr, A Kraus, S Grampp, M Wiesener, M Schiffer, A Hartmann, B Wullich, J Schödel
    The Journal of Biological Chemistry, 2022-02-08;0(0):101699.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Adenylate Kinase 4—A Key Regulator of Proliferation and Metabolic Shift in Human Pulmonary Arterial Smooth Muscle Cells via Akt and HIF-1 alpha Signaling Pathways
    Authors: Magdalena Wujak, Christine Veith, Cheng-Yu Wu, Tessa Wilke, Zeki Ilker Kanbagli, Tatyana Novoyatleva et al.
    International Journal of Molecular Sciences
  8. Targeting HIF-activated collagen prolyl 4-hydroxylase expression disrupts collagen deposition and blocks primary and metastatic uveal melanoma growth
    Authors: S Kaluz, Q Zhang, Y Kuranaga, H Yang, S Osuka, D Bhattachar, NS Devi, J Mun, W Wang, R Zhang, MM Goodman, HE Grossnikla, EG Van Meir
    Oncogene, 2021-07-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Modified Hypoxia-Inducible Factor Expression in CD8(+) T Cells Increases Antitumor Efficacy
    Authors: Veli�a P, Cunha PP, Vojnovic N et al.
    Cancer Immunology Research
  10. Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity
    Authors: W Yang, T Yu, X Huang, AJ Bilotta, L Xu, Y Lu, J Sun, F Pan, J Zhou, W Zhang, S Yao, CL Maynard, N Singh, SM Dann, Z Liu, Y Cong
    Nat Commun, 2020-09-08;11(1):4457.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions
    Authors: K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama
    J Neuroinflammation, 2020-05-02;17(1):141.
    Species: Rat
    Sample Types: Cell Lysate
    Applications: Western Blot
  12. TET-Mediated Hypermethylation Primes SDH-Deficient Cells for HIF2&alpha-Driven Mesenchymal Transition
    Authors: A Morin, J Goncalves, S Moog, LJ Castro-Veg, S Job, A Buffet, MJ Fontenille, J Woszczyk, AP Gimenez-Ro, E Letouzé, J Favier
    Cell Rep, 2020-03-31;30(13):4551-4566.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

  13. Ascorbate modulates the hypoxic pathway by increasing intracellular activity of the HIF hydroxylases in renal cell carcinoma cells
    Authors: Christina Wohlrab, Caroline Kuiper, Margreet CM Vissers, Elisabeth Phillips, Bridget A Robinson, Gabi U Dachs
    Hypoxia (Auckl)
  14. The lncRNA Neat1 promotes activation of inflammasomes in macrophages
    Authors: P Zhang, L Cao, R Zhou, X Yang, M Wu
    Nat Commun, 2019-04-02;10(1):1495.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Suppressive effects of iron chelation in clear cell renal cell carcinoma and their dependency on VHL inactivation
    Authors: Christopher J. Greene, Nitika J. Sharma, Peter N. Fiorica, Emily Forrester, Gary J. Smith, Kenneth W. Gross et al.
    Free Radical Biology and Medicine
  16. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
    Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
    PLoS ONE, 2018-12-19;13(12):e0208579.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Astrocyte HIF-2α supports learning in a passive avoidance paradigm under hypoxic stress
    Authors: Cindy V Leiton, Elyssa Chen, Alissa Cutrone, Kristy Conn, Kennelia Mellanson, Dania M Malik et al.
    Hypoxia (Auckl)
  18. The Association Between Ascorbate and the Hypoxia-Inducible Factors in Human Renal Cell Carcinoma Requires a Functional Von Hippel-Lindau Protein
    Authors: Christina Wohlrab, Margreet C. M. Vissers, Elisabeth Phillips, Helen Morrin, Bridget A. Robinson, Gabi U. Dachs
    Frontiers in Oncology
  19. Modeling Renal Cell Carcinoma in Mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade
    Authors: Yi-Feng Gu, Shannon Cohn, Alana Christie, Tiffani McKenzie, Nicholas Wolff, Quyen N. Do et al.
    Cancer Discovery
  20. Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin
    Authors: H Kobayashi, Q Liu, TC Binns, AA Urrutia, O Davidoff, PP Kapitsinou, AS Pfaff, H Olauson, A Wernerson, AB Fogo, GH Fong, KW Gross, VH Haase
    J Clin Invest, 2016-04-18;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Unprocessed Interleukin-36alpha Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1.
    Authors: Milora K, Fu H, Dubaz O, Jensen L
    J Invest Dermatol, 2015-07-23;135(12):2992-3000.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  22. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
    Authors: Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson T, Rankin E, Giaccia A, Schipani E
    Dev Biol, 2014-06-24;393(1):124-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions.
    Authors: Swain L, Wottawa M, Hillemann A, Beneke A, Odagiri H, Terada K, Endo M, Oike Y, Farhat K, Katschinski D
    J Leukoc Biol, 2014-03-13;96(3):365-75.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. HIF-1alpha activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.
    Authors: Weidemann A, Breyer J, Rehm M, Eckardt K, Daniel C, Cicha I, Giehl K, Goppelt-Struebe M
    Cell Commun Signal, 2013-10-21;11(0):80.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. IL-4 reduces the proangiogenic capacity of macrophages by down-regulating HIF-1alpha translation.
    Authors: Dehne N, Tausendschon M, Essler S, Geis T, Schmid T, Brune B
    J Leukoc Biol, 2013-09-04;95(1):129-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-beta1 processing.
    Authors: Wottawa M, Leisering P, Ahlen M, Schnelle M, Vogel S, Malz C, Bordoli M, Camenisch G, Hesse A, Napp J, Alves F, Kristiansen G, Farhat K, Katschinski D
    Int J Cancer, 2012-12-27;132(12):2787-98.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Hypoxia-inducible Factor-1 (HIF-1) but Not HIF-2 Is Essential for Hypoxic Induction of Collagen Prolyl 4-Hydroxylases in Primary Newborn Mouse Epiphyseal Growth Plate Chondrocytes.
    Authors: Aro E, Khatri R
    J. Biol. Chem., 2012-08-28;287(44):37134-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
    Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
    BMC Cancer, 2012-05-29;12(0):201.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Differential activation and antagonistic function of HIF-{alpha} isoforms in macrophages are essential for NO homeostasis
    Authors: Norihiko Takeda, Ellen L. O'Dea, Andrew Doedens, Jung-whan Kim, Alexander Weidemann, Christian Stockmann et al.
    Genes & Development
  30. Loss of myeloid cell-derived vascular endothelial growth factor accelerates fibrosis.
    Authors: Stockmann C, Kerdiles Y, Nomaksteinsky M, Weidemann A, Takeda N, Doedens A, Torres-Collado AX, Iruela-Arispe L, Nizet V, Johnson RS
    Proc. Natl. Acad. Sci. U.S.A., 2010-02-08;107(9):4329-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Hepatic hepcidin/intestinal HIF-2a axis maintains iron absorption during iron deficiency and overload.
    Authors: Schwartz AJ, Das Nk, Ramakrishnan Sk et al.
    J. Clin. Invest.
  32. PHD2 Is a Regulator for Glycolytic Reprogramming in Macrophages.
    Authors: Guentsch A, Beneke A, Swain L et al.
    Mol. Cell. Biol.
  33. Endothelial Cell HIF-1[alpha] and HIF-2[alpha] Differentially Regulate Metastatic Success.
    Authors: Branco-Price C, Zhang N, Schnelle M et al.
    Cancer Cell 1721(1):52-65.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody and earn rewards!

Have you used Human/Mouse/Rat HIF-2 alpha /EPAS1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review