Human/Mouse/Rat HSPB8 Antibody Summary
Ala2-Thr196
Accession # Q9UJY1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat HSPB8 by Western Blot. Western blot shows lysates of human, mouse, and rat heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPB8 Monoclonal Antibody (Catalog # MAB4987) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPB8 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSPB8
Heat shock protein beta-8 (HSPB8, also HSP22 or CRYAC) is a 22‑25 kDa member of the small HSP (HSP20) family of proteins. It is expressed in muscle (smooth, skeletal and cardiac), and serves as a molecular chaperone. Human HSPB8 contains one alpha -crystalline domain (aa 93‑170) that mediates protein-protein interaction, and N- plus C-terminal flanking sequences that generate homodimers, homooligomers, and heterodimers with HSP27 and HSPB7. Phosphorylation at Ser57 blocks HSPB8 chaperone activity. Human HSPB8 shares 94% aa identity with both mouse and canine HSPB8. Deficiencies in HSPB8 are involved in distal motor neuropathy type 11A.
Product Datasheets
Citations for Human/Mouse/Rat HSPB8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Bicalutamide and Trehalose Ameliorate Spinal and Bulbar Muscular Atrophy Pathology in Mice
Authors: Mariarita Galbiati, Marco Meroni, Marina Boido, Matilde Cescon, Paola Rusmini, Valeria Crippa et al.
Neurotherapeutics
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HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy
Authors: R Cristofani, M Piccolella, M Montagnani, B Tedesco, A Poletti, RM Moretti
Cell Death & Disease, 2022-11-18;13(11):973.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Correlation between the complex of small heat shock proteins (HSPBs) and the progression in patients with hepatocellular carcinoma
Authors: R Matsushima, H Toyoda, M Atsuyuki, Y Kaneoka, T Kumada, O Kozawa
Archives of biochemistry and biophysics, 2022-11-05;732(0):109461.
Species: Human
Sample Types: Tissue Homogenates
Applications: Co-Immunoprecipitation, Western Blot -
HSPB8 Promotes the Fusion of Autophagosome and Lysosome during Autophagy in Diabetic Neurons
Authors: XC Li, QK Hu, L Chen, SY Liu, S Su, H Tao, LN Zhang, T Sun, LJ He
Int J Med Sci, 2017-10-15;14(13):1335-1341.
Species: Human, Rat
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle
Authors: Ulbricht A, Gehlert S, Leciejewski B et al.
Autophagy.
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