Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody Summary
5% cross-reactivity with recombinant mouse IMPDH2 is observed.
Met1-Tyr563 (predicted)
Accession # P20839
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse IMP Dehydrogenase 1/IMPDH1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IMP Dehydrogenase 1/IMPDH1 at approximately 55-57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IMP Dehydrogenase 1/IMPDH1 in HeLa Human Cervical Epithelial Carcinoma Cell Line. IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at a 1:200 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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IMP Dehydrogenase 1/IMPDH1 in C2C12 Mouse Cell Line. IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human IMP Dehydrogenase 1/IMPDH1 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IMP Dehydrogenase 1/IMPDH1 at approximately 56 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IMP Dehydrogenase 1/IMPDH1
The enzyme IMPDH1 (Inosine-5'-monophosphate dehydrogenase 1) catalyzes the conversion of inosine
5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed
and rate-limiting step in the de novo synthesis of guanine nucleotides,
and therefore plays an important role in the regulation of cell growth.The enzyme is 514 aminoacids long, with a predicted molecular weight of 55 kDa.
Product Datasheets
Product Specific Notices
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.FAQs
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