Human/Mouse/Rat JNK Pan Specific Antibody Summary
Met1-Gln384
Accession # P45983
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat JNK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of recombinant JNK family members by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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JNK in Human Prostate Cancer Tissue. JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
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JNK in Rat Kidney. JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Human JNK by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JNK
The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.
Product Datasheets
Citations for Human/Mouse/Rat JNK Pan Specific Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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Pressure stress delays cyclooxygenase-2 expression induced by interleukin-1? in cultured human pulmonary artery smooth muscle cells
Authors: Hiraide, S;Machida, T;Takihana, S;Ohshita, M;Iizuka, K;
Heliyon
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth
Authors: Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz
iScience
Species: Transgenic Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
Authors: Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li
iScience
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Dysregulated splicing factor SF3B1 unveils a dual therapeutic vulnerability to target pancreatic cancer cells and cancer stem cells with an anti-splicing drug
Authors: Emilia Alors-Perez, Ricardo Blázquez-Encinas, Sonia Alcalá, Cristina Viyuela-García, Sergio Pedraza-Arevalo, Vicente Herrero-Aguayo et al.
Journal of Experimental & Clinical Cancer Research
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Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids
Authors: J Aguado, HK Chaggar, C Gómez-Incl, MR Shaker, HC Leeson, A Mackay-Sim, EJ Wolvetang
Aging Cell, 2021-08-30;0(0):e13468.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
Authors: J Falero-Per, N Sheibani, CM Sorenson
PLoS ONE, 2020-05-04;15(5):e0232779.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer
Authors: JM Jiménez-Va, V Herrero-Ag, AJ Montero-Hi, E Gómez-Góme, AC Fuentes-Fa, AJ León-Gonzá, P Sáez-Martí, E Alors-Pére, S Pedraza-Ar, T González-S, O Reyes, A Martínez-L, R Sánchez-Sá, S Ventura, EM Yubero-Ser, MJ Requena-Ta, JP Castaño, MD Gahete, RM Luque
EBioMedicine, 2020-01-03;51(0):102547.
Species: Human
Sample Types: Cell Cultue Supernates
Applications: Western Blot -
Role of the MAPK/cJun NH2-Terminal Kinase signaling pathway in starvation-induced autophagy
Authors: SA Barutcu, N Girnius, S Vernia, RJ Davis
Autophagy, 2018-08-17;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The cJUN NH2-terminal kinase (JNK) signaling pathway promotes genome stability and prevents tumor initiation
Authors: Nomeda Girnius, Yvonne JK Edwards, David S Garlick, Roger J Davis
eLife
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JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins
Authors: N Girnius, RJ Davis
Cell Rep, 2017-11-14;21(7):1910-1921.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells
Authors: L Courtial, V Picco, R Grover, Y Cormerais, C Rottier, A Labbe, G Pagès, C Ferrier-Pa
Sci Rep, 2017-04-04;7(0):45713.
Species: Coral - Stylophora pistillata
Sample Types: Tissue Homogenates
Applications: Western Blot -
White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
Nat Cell Biol, 2014-12-08;17(1):57-67.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
Authors: Lopez R, Demick KP, Mansfield JM, Paulnock DM
J. Immunol., 2008-10-01;181(7):4908-17.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor.
Authors: Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S
J. Interferon Cytokine Res., 2008-04-01;28(4):235-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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