Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antibody
Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antibody Summary
Ala2-Val92
Accession # P00338
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Lactate Dehydrogenase A/LDHA by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, mouse skeletal muscle tissue, and rat skeletal muscle tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7304) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lactate Dehydrogenase A/LDHA at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Lactate Dehydrogenase A/LDHA by Simple WesternTM. Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Lactate Dehydrogenase A/LDHA at approximately 38 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7304). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human Lactate Dehydrogenase A/LDHA by Simple WesternTM. Simple Western shows lysates of Exosome Standards (HEK293 cell line) (NBP3-11684) and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Lactate Dehydrogenase A/LDHA at approximately 38 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7304). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lactate Dehydrogenase A/LDHA
LDHA (lactate dehydrogenase A chain; also LDH-M and PIG-19) is a 34-36 kDa member of the LDH family of enzymes. It is a part of a cytoplasmic complex that is found principally in hepatocytes and skeletal muscle. The LDHA complex catalyzes the conversion of pyruvate to lactate, thereby generating NAD, the final step in anerobic glycolysis. This NAD is essential for the subsequent generation of ATP. Human LDHA is 332 amino acids (aa) in length. It contains an N-terminal coenzyme binding region, a central catalytic site, and at least nine utilized Lys acetylation and two Tyr phosphorylation sites. It also undergoes ISGylation where a 17 kDa product of the ISG15 gene is covalently attached to LDHA in a ubiquitin-like manner. LDHA forms tetramers composed of two dimers of varying composition. The tetramer may be homotetrameric (four monomers), or contain from one to three substitute LDHB monomers typically found in heart muscle. There are multiple splice variants. One possesses a five aa substitution for aa 237-332, a second contains a 45 aa substitution for aa 230-332, a third shows a deletion of aa 82-139, while a fourth utilizes an alternative start site 29 aa upstream of the standard site. Over aa 2-92, human LDHA shares 93% aa sequence identity with mouse LDHA.
Product Datasheets
Citations for Human/Mouse/Rat Lactate Dehydrogenase A/LDHA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasion
Authors: Yijie Ren, Anil Kumar, Jugal K Das, Hao-Yun Peng, Liqing Wang, Darby Balllard et al.
Journal for ImmunoTherapy of Cancer
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Metformin suppresses Nrf2-mediated chemoresistance in hepatocellular carcinoma cells by increasing glycolysis
Authors: Liangyu Cai, Xin Jin, Jiannan Zhang, Le Li, Jinfeng Zhao
Aging (Albany NY)
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Lactate dehydrogenase inhibition synergizes with IL-21 to promote CD8+ T cell stemness and antitumor immunity
Authors: D Hermans, S Gautam, JC García-Cañ, D Gromer, S Mitra, R Spolski, P Li, S Christense, R Nguyen, JX Lin, J Oh, N Du, S Veenbergen, J Fioravanti, R Ebina-Shib, C Bleck, LM Neckers, JD Rabinowitz, L Gattinoni, WJ Leonard
Proc. Natl. Acad. Sci. U.S.A., 2020-03-02;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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