Human/Mouse/Rat MBP Antibody Summary
Accession # P02686
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MBP by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse and Rat MBP by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MBP in Rat Cortical Stem Cells. MBP was detected in immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces, cytoplasm, and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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MBP in Human Brain. MBP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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MBP in Mouse Brain. MBP was detected in perfusion fixed frozen sections of mouse brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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MBP in Rat Brain. MBP was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MBP
Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.
Product Datasheets
Citations for Human/Mouse/Rat MBP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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The major TMEM106B dementia risk allele affects TMEM106B protein levels, fibril formation, and myelin lipid homeostasis in the ageing human hippocampus
Authors: Lee JY, Harney DJ, Teo JD et al.
Mol Neurodegener
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Injury-induced perivascular niche supports alternative differentiation of adult rodent CNS progenitor cells
Authors: Justyna Ulanska-Poutanen, Jakub Mieczkowski, Chao Zhao, Katarzyna Konarzewska, Beata Kaza, Hartmut BF Pohl et al.
eLife
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A novel 3D bilayer hydrogel tri-culture system for studying functional motor units
Authors: Yu-Lung Lin, Jennifer Nhieu, Thomas Lerdall, Liming Milbauer, Chin-Wen Wei, Dong Jun Lee et al.
Cell Biosci
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Vitamin C regulates Schwann cell myelination by promoting DNA demethylation of pro‐myelinating genes
Authors: Tyler C. Huff, David W. Sant, Vladimir Camarena, Derek Van Booven, Nadja S. Andrade, Sushmita Mustafi et al.
Journal of Neurochemistry
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The major TMEM106B dementia risk allele affects TMEM106B protein levels, fibril formation, and myelin lipid homeostasis in the ageing human hippocampus
Authors: Lee JY, Harney DJ, Teo JD et al.
Mol Neurodegener
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Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain
Authors: You Y, Muraoka S, Jedrychowski MP et al.
Journal of Extracellular Vesicles
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Microglial lysosome dysfunction contributes to white matter pathology and TDP-43 proteinopathy in GRN-associated FTD
Authors: Y Wu, W Shao, TW Todd, J Tong, M Yue, S Koga, M Castanedes, AL Librero, CW Lee, IR Mackenzie, DW Dickson, YJ Zhang, L Petrucelli, M Prudencio
Cell Reports, 2021-08-24;36(8):109581.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Quercetin Attenuates Diabetic Peripheral Neuropathy by Correcting Mitochondrial Abnormality via Activation of AMPK/PGC-1&alpha Pathway in vivo and in vitro
Authors: Q Zhang, W Song, B Zhao, J Xie, Q Sun, X Shi, B Yan, G Tian, X Liang
Frontiers in Neuroscience, 2021-03-03;15(0):636172.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Early Reperfusion Following Ischemic Stroke Provides Beneficial Effects, Even After Lethal Ischemia with Mature Neural Cell Death
Authors: Y Tanaka, N Nakagomi, N Doe, A Nakano-Doi, T Sawano, T Takagi, T Matsuyama, S Yoshimura, T Nakagomi
Cells, 2020-06-01;9(6):.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: IHC -
5-HT1a activation in PO/AH area induces therapeutic hypothermia in a rat model of intracerebral hemorrhage
Authors: T Liang, Q Chen, Q Li, R Li, J Tang, R Hu, J Zhong, H Ge, X Liu, F Hua
Oncotarget, 2017-08-16;8(43):73613-73626.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain
Authors: You Y, Muraoka S, Jedrychowski MP et al.
Journal of Extracellular Vesicles
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