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Human/Mouse/Rat MDM2/HDM2 Antibody

Catalog #: AF1244
21 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1244
AF1244-SP
MDM2/HDM2 in Human Kidney.
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Product Details
Citations (21)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat MDM2/HDM2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat MDM2.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MDM2
Asn3-Pro491
Accession # Q00987
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

MDM2/HDM2 in Human Kidney. MDM2/HDM2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse/Rat MDM2/HDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei for epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry MDM2 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

MDM2 in Mouse Kidney. MDM2 was detected in perfusion fixed frozen sections of mouse kidney using Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human MDM2 antibody by Western Blot. View Larger

Detection of Human MDM2 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, and CEM human T-lymphoblastoid cell line untreated (-) or treated (+) with 5 µM LLnV for 1 hour. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDM2/HDM2

MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally up‑regulates the expression of its own inhibitor, MDM2.

Long Name
Double Minute 2 Protein
Entrez Gene IDs
4193 (Human); 17246 (Mouse)
Alternate Names
ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2

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Citations for Human/Mouse/Rat MDM2/HDM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. The MDM2-p53 Axis Represents a Therapeutic Vulnerability Unique to Glioma Stem Cells
    Authors: Nakagawa-Saito, Y;Mitobe, Y;Togashi, K;Suzuki, S;Sugai, A;Takenouchi, S;Nakamura, K;Sonoda, Y;Kitanaka, C;Okada, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Antagonizing MDM2 Overexpression Induced by MDM4 Inhibitor CEP-1347 Effectively Reactivates Wild-Type p53 in Malignant Brain Tumor Cells
    Authors: Yuta Mitobe, Shuhei Suzuki, Yurika Nakagawa-Saito, Keita Togashi, Asuka Sugai, Yukihiko Sonoda et al.
    Cancers (Basel)
  3. Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
    Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
    Biosensors
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: IHC
  4. The Novel MDM4 Inhibitor CEP-1347 Activates the p53 Pathway and Blocks Malignant Meningioma Growth In Vitro and In Vivo
    Authors: Mitobe, Y;Suzuki, S;Nakagawa-Saito, Y;Togashi, K;Sugai, A;Sonoda, Y;Kitanaka, C;Okada, M;
    Biomedicines
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot
  5. Cardiac GRK2 Protein Levels Show Sexual Dimorphism during Aging and Are Regulated by Ovarian Hormones
    Authors: AC Arcones, MR Martínez-C, R Vila-Bedma, C Yáñez, I Lladó, AM Proenza, F Mayor, C Murga
    Cells, 2021-03-17;10(3):.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Targeting Triple Negative Breast Cancer with a Nucleus-Directed p53 Tetramerization Domain Peptide
    Authors: Gu Xiao, George K. Annor, Kimberly Fung, Outi Keinänen, Brian M. Zeglis, Jill Bargonetti
    Molecular Pharmaceutics
  7. Molecular Targeting of H/MDM-2 Oncoprotein in Human Colon Cancer Cells and Stem-like Colonic Epithelial-derived Progenitor Cells
    Authors: ANUSHA THADI, WILLIAM F. MORANO, MARIAN KHALILI, BLAKE D. BABCOCK, MOHAMMAD F. SHAIKH, DESHKA S. FOSTER et al.
    Anticancer Research
  8. Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation
    Authors: A Colmenero-, MA Gómez-Muño, I Rodríguez-, A Amador-Álv, KO Henrich, D Pascual-Va, K Okonechnik, E Rivas, F Westermann, R Pardal, FM Vega
    Cancers (Basel), 2020-11-20;12(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Targeting Membrane HDM-2 by PNC-27 Induces Necrosis in Leukemia Cells But Not in Normal Hematopoietic Cells
    Authors: ANUSHA THADI, LAUREN LEWIS, EVE GOLDSTEIN, ANSHU AGGARWAL, MARIAN KHALILI, LINDSAY STEELE et al.
    Anticancer Research
  10. Dual Antagonist of cIAP/XIAP ASTX660 Sensitizes HPV(−) and HPV(+) Head and Neck Cancers To TNF alpha, TRAIL, and Radiation Therapy
    Authors: Roy Xiao, Yi An, Wenda Ye, Adeeb Derakhshan, Hui Cheng, Xinping Yang et al.
    Clinical Cancer Research
  11. The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage
    Authors: Eutteum Jeong, Eutteum Brady, José A Martina, Mehdi Pirooznia, Ilker Tunc, Rosa Puertollano
    eLife
  12. A novel mouse model of rhabdomyosarcoma underscores the dichotomy of MDM2-ALT1 function in vivo
    Authors: Daniel F. Comiskey Jr, Aishwarya G. Jacob, Brianne L. Sanford, Matías Montes, Andrew K. Goodwin, Haley Steiner et al.
    Oncogene
  13. Adverse Effects on ?-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function
    Authors: R Schreckenb, P Bencsik, M Weber, Y Abdallah, C Csonka, K Gömöri, K Kiss, J Pálóczi, J Pipis, M Sárközy, P Ferdinandy, R Schulz, KD Schlüter
    J Am Heart Assoc, 2017-12-22;6(12):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
    Authors: Phesse, T J, Myant, K B, Cole, A M, Ridgway, R A, Pearson, H, Muncan, V, van den Brink, G R, Vousden, K H, Sears, R, Vassilev, L T, Clarke, A R, Sansom, O J
    Cell Death Differ, 2014-02-28;21(6):956-66.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
    J Hematol Oncol, 2012-09-18;5(0):57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells.
    Authors: Surget S, Chiron D, Gomez-Bougie P, Descamps G, Menoret E, Bataille R, Moreau P, Le Gouill S, Amiot M, Pellat-Deceunynck C
    Cancer Res, 2012-06-27;72(17):4562-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Sudemycins, Novel Small Molecule Analogues of FR901464, Induce Alternative Gene Splicing
    Authors: Liying Fan, Chandraiah Lagisetti, Carol C. Edwards, Thomas R. Webb, Philip M. Potter
    ACS Chemical Biology
  19. Anoikis triggers Mdm2-dependent p53 degradation
    Authors: Abhijit Ghosh, Tina Chunyuan Chen, Yvonne L. Kapila
    Molecular and Cellular Biochemistry
  20. A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development.
    Authors: Post SM, Quintas-Cardama A, Pant V, Iwakuma T, Hamir A, Jackson JG, Maccio DR, Bond GL, Johnson DG, Levine AJ, Lozano G
    Cancer Cell, 2010-09-14;18(3):220-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
    Authors: Rozan LM, McDonald ER, Navaraj A, Matthew EM, Dicker DT, El-Deiry WS
    J. Biol. Chem., 2006-11-22;282(5):3273-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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