Human/Mouse/Rat p130Cas Antibody Summary
Met66-Val412
Accession # P56945
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat p130Cas by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human p130Cas Monoclonal Antibody (Catalog # MAB5730) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p130Cas at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p130Cas
p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105-130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Pro‑rich region (aa 74-87), at least 10 phosphotyrosine (aa 128-410) and four phosphoserine (aa 134-639) sites, an SH3 binding motif (aa 635-643) and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22 aa substitution for aa 1-4, an alternate start site at Met30, a deletion of aa 2-211, and a four aa substitution for aa 1-4 with a deletion of aa 65-212. Over aa 66-412, human p130Cas shares 91% aa identity with mouse p130Cas.
Product Datasheets
Citation for Human/Mouse/Rat p130Cas Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Conditional deletion of CD98hc inhibits osteoclast development
Authors: Hideki Tsumura, Morihiro Ito, Masamichi Takami, Miyuki Arai, Xiao-Kang Li, Toshio Hamatani et al.
Biochemistry and Biophysics Reports
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