Human/Mouse/Rat p27/Kip1 Antibody

Name: Human/Mouse/Rat p27/Kip1 Antibody
Brand: R&D Systems
SKU: MAB22561
Rating: 5 (1 reviews)

Catalog #: MAB22561 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB22561
MAB22561-SP

p27/Kip1 in Human Prostate Cancer Tissue.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat p27/Kip1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human p27/Kip1 in direct ELISAs. Specificity was demonstrated by comparing staining on tissue sections of normal versus p27/Kip1 knockout mice.

Source

Monoclonal Rat IgG_2A Clone # 341116

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human p27/Kip1
Met1-Thr198
Accession # P46527

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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p27/Kip1 in Human Prostate Cancer Tissue. p27/Kip1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Rat Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB22561) overnight at 4 °C. Tissue was stained with the Human p27 Immunohistochemistry Detection Kit (brown; Catalog # CTS2256) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. Human p27/Kip1 shares 88% and 87% amino acid sequence identity with mouse and rat p27/Kip1, respectively, while mouse and rat p27/Kip1 share 96% amino acid sequence identity.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Entrez Gene IDs

1027 (Human); 12576 (Mouse); 83571 (Rat)

Alternate Names

CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1