Human/Mouse/Rat Paxillin Antibody Summary
Asn59-Ser274
Accession # P49023
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Paxillin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Paxillin in HUVEC Human Cells. Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips and cytosol. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Paxillin by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Paxillin at approximately 70 & 69 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Paxillin
The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased Paxillin protein levels have also been reported in several malignancies, including renal, lung, and breast carcinomas.
Product Datasheets
Citations for Human/Mouse/Rat Paxillin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells
Authors: Chen Y, Tristan CA, Lu C et al.
Nat Methods
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Cas phosphorylation regulates focal adhesion assembly
Authors: Saurav Kumar, Amanda Stainer, Julien Dubrulle, Christopher Simpkins, Jonathan A Cooper
eLife
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A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics
Authors: Elizabeth M Steenkiste, Jason D Berndt, Carissa Pilling, Christopher Simpkins, Jonathan A Cooper
eLife
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Platelet-derived growth factor receptor-alpha and Ras-related C3 botulinum toxin substrate-1 regulate mechano-responsiveness of lung fibroblasts
Authors: Stephen E. McGowan, Diann M. McCoy
American Journal of Physiology-Lung Cellular and Molecular Physiology
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CAF-like state in primary skin fibroblasts with constitutional BRCA1 epimutation sheds new light on tumor suppressor deficiency-related changes in healthy tissue.
Authors: Etzold A, Galetzka D, Weis E, Bartsch O, Haaf T, Spix C, Itzel T, Schweiger S, Strand D, Strand S, Zechner U
Epigenetics, 2016-03-07;11(2):120-31.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Mechanoresponsive, omni-directional and local matrix-degrading actin protrusions in human mesenchymal stem cells microencapsulated in a 3D collagen matrix.
Authors: Ho F, Zhang W, Li Y, Chan B
Biomaterials, 2015-03-19;53(0):392-405.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis
Authors: Antoniades I, Kyriakou M, Charalambous A, et al.
Cell communication and signaling : CCS
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