Human/Mouse/Rat PBEF/Visfatin Antibody Summary
Pro27-His491
Accession # P43790
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat PBEF/Visfatin by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/ macrophage cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PBEF/Visfatin by Western Blot. Western blot shows lysates of human heart tissue, 293T human embryonic kidney cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/ Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PBEF/Visfatin by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/ Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human PBEF/Visfatin by Simple WesternTM. Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for PBEF/Visfatin at approximately 58 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB40441). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human and Mouse PBEF/Visfatin by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PBEF/Visfatin at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PBEF/Visfatin
PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.
Product Datasheets
Citation for Human/Mouse/Rat PBEF/Visfatin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
Nature Communications, 2022-11-25;13(1):7269.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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